Date published: 2025-10-5

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Huntingtin Antibody (3E10): sc-47757

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Datasheets
  • Huntingtin Antibody (3E10) is a mouse monoclonal IgG1 κ Huntingtin antibody, cited in 5 publications, provided at 200 µg/ml
  • raised against amino acids 997-1276 of Huntingtin of mouse origin
  • Huntingtin Antibody (3E10) is recommended for detection of an epitope corresponding to the HDA region (amino acids 1171-1177) of Huntingin of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • Anti-Huntingtin Antibody (3E10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Huntingtin Antibody (3E10). This work is in progress.

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Huntingtin Antibody (3E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Huntingtin (HTT) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-HTT antibody (3E10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HTT (3E10) monoclonal antibody plays a crucial role in studying neuronal function, intracellular transport, transcriptional regulation, and synaptic function. HTT′s proper functioning is vital for maintaining neuronal health, and mutations in HTT, particularly those leading to an expanded polyglutamine region, are associated with Huntington′s disease. The abnormal polyglutamine expansion results in a toxic gain of function, leading to neurodegeneration through mechanisms that may involve altered interactions with other cellular proteins. Notably, HTT interacts with several key proteins, including HAP1, glyceraldehyde phosphate dehydrogenase (GAPDH), and HIP1, which are implicated in various cellular processes. Understanding these interactions is essential for elucidating the pathophysiology of Huntington′s disease and developing potential therapeutic strategies.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Huntingtin Antibody (3E10) References:

  1. A huntingtin-associated protein enriched in brain with implications for pathology.  |  Li, XJ., et al. 1995. Nature. 378: 398-402. PMID: 7477378
  2. Genetics and molecular biology of Huntington's disease.  |  Albin, RL. and Tagle, DA. 1995. Trends Neurosci. 18: 11-4. PMID: 7535483
  3. Structure and expression of the Huntington's disease gene: evidence against simple inactivation due to an expanded CAG repeat.  |  Ambrose, CM., et al. 1994. Somat Cell Mol Genet. 20: 27-38. PMID: 8197474
  4. A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. The Huntington's Disease Collaborative Research Group.  |  . 1993. Cell. 72: 971-83. PMID: 8458085
  5. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH.  |  Burke, JR., et al. 1996. Nat Med. 2: 347-50. PMID: 8612237
  6. HIP1, a human homologue of S. cerevisiae Sla2p, interacts with membrane-associated huntingtin in the brain.  |  Kalchman, MA., et al. 1997. Nat Genet. 16: 44-53. PMID: 9140394
  7. Huntington's disease.  |  Gusella, JF., et al. 1996. Cold Spring Harb Symp Quant Biol. 61: 615-26. PMID: 9246488
  8. Huntingtin acts in the nucleus to induce apoptosis but death does not correlate with the formation of intranuclear inclusions.  |  Saudou, F., et al. 1998. Cell. 95: 55-66. PMID: 9778247

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Huntingtin Antibody (3E10)

sc-47757
200 µg/ml
$316.00

Huntingtin Antibody (3E10) AC

sc-47757 AC
500 µg/ml, 25% agarose
$416.00

Huntingtin Antibody (3E10) HRP

sc-47757 HRP
200 µg/ml
$316.00

Huntingtin Antibody (3E10) FITC

sc-47757 FITC
200 µg/ml
$330.00

Huntingtin Antibody (3E10) PE

sc-47757 PE
200 µg/ml
$343.00

Huntingtin Antibody (3E10) Alexa Fluor® 488

sc-47757 AF488
200 µg/ml
$357.00

Huntingtin Antibody (3E10) Alexa Fluor® 546

sc-47757 AF546
200 µg/ml
$357.00

Huntingtin Antibody (3E10) Alexa Fluor® 594

sc-47757 AF594
200 µg/ml
$357.00

Huntingtin Antibody (3E10) Alexa Fluor® 647

sc-47757 AF647
200 µg/ml
$357.00

Huntingtin Antibody (3E10) Alexa Fluor® 680

sc-47757 AF680
200 µg/ml
$357.00

Huntingtin Antibody (3E10) Alexa Fluor® 790

sc-47757 AF790
200 µg/ml
$357.00

The Huntingtin Antibody (3E10): sc-47757 links to the N-Terminal of the protein?

Asked by: Carina
Thank you for your question. Huntingtin Antibody (3E10): sc-47757 was raised against amino acids 997-1276 of Huntingtin of mouse origin.
Answered by: Tech Support Europe
Date published: 2023-12-14

Can I get a quote about Huntingtin Antibody (3E10): sc-47757?

Asked by: zcczcw
Thank you for your question. For accurate availability, shipping and pricing information regarding our products, or to receive a quote, please contact Customer Service.
Answered by: Tech Service
Date published: 2020-03-12

I am interested in the FREE 10 µg sample of Huntingtin (3E10): sc-47757. I want yo know what is the procedure for this request

Asked by: MrMauricio
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using Huntingtin (3E10): sc-47757 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good!This produced clean and specific signals in WB (5% milk, 1:200)
Date published: 2018-11-13
Rated 4 out of 5 by from Good AntibodyI tested this antibody on mouse brain lysate. I detected two major protein bands in WB (1:200 dilution). One protein band is around 400 kDa and another one is 130 kDa protein band.
Date published: 2018-07-09
Rated 5 out of 5 by from Good for WBI have tested by WB, 1:1000 (blocking milk 5% and primary Ab dilution in BSA 3% ON) and it worked very well for both endogenous protein and recombinant HTT.
Date published: 2017-11-07
Rated 4 out of 5 by from Works wellWe have this antibody in our lab and found it works well.
Date published: 2017-04-05
Rated 5 out of 5 by from western blottingUsed for western blotting for mouse liver tissue analysis , the antibody can work well in 1:500, strong signal.
Date published: 2017-03-18
Rated 4 out of 5 by from Potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~350 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for medium immunoreactivity with a ~100 kDa protein.
Date published: 2016-11-10
Rated 4 out of 5 by from Wonderful immunoperoxidase cytoplasmic stainingWonderful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-04-24
Rated 4 out of 5 by from Single strong band at correct mw in JurkatSingle strong band at correct mw in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-05-22
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Huntingtin Antibody (3E10) is rated 4.4 out of 5 by 8.
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