HuD Antibody (H-9) recommended for detection of HuD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HuD Antibody (H-9): sc-48421

HuD Antibody (H-9) is rated 5.0 out of 5 by 9.
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Datasheets

    • Anti-HuD Antibody (H-9) is a mouse monoclonal IgG2a (kappa light chain) HuD antibody provided at 200 µg/ml
    • raised against amino acids 1-300 of HuD of human origin
    • Anti-HuD Antibody (H-9) is recommended for detection of HuD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HuD Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HuD (H-9): sc-48421.
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HuD Antibody (H-9): sc-48421: Can you please provide the clone ID information?

Asked by: Leena Kumar
Thank you very much for your question. We produced this antibody against a recomb. protein fragment consisting of amino acids 1-300 of HuD of human origin. The same antigen was used for the other currently available antibody. https://www.scbt.com/scbt/browse/hud-Antibodies/_/N-11n1jcq The exact binding epitope for this monoclonal antibody has not been mapped, yet.
Answered by: Technical Support Europe
Date published: 2018-01-16

What is the total volume provided of HuD (H-9):sc-48421 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-13

Can HuD (H-9): sc-48421 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, HuD (H-9): sc-48421 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from Strong signal Has good signal for paraffin-embedded sample for fluorescent immunohistochemistry.
Date published: 2017-05-16
Rated 5 out of 5 by from Superb for multiple applications !! Great IHC staining results; also strong WB; much better than polyclonal alternatives
Date published: 2017-01-19
Rated 5 out of 5 by from Great antibody for staining This HuD antibody shows good results for IHC in human tissue.
Date published: 2017-01-12
Rated 5 out of 5 by from great antibody I tried this as a sample antibody from Santa Cruz and it really worked well on mouse intestine cryo sections at 1/ 400 dilution for myenteric ganglia.
Date published: 2016-12-23
Rated 5 out of 5 by from Working well in IF in zebrafish Working well in IF in zebrafish.
Date published: 2016-08-08
Rated 5 out of 5 by from Were able to publish for use in IF in rat tissue Were able to publish for use in IF in rat tissue.
Date published: 2015-06-04
Rated 5 out of 5 by from Positive signal in WB in SK Positive signal in WB in SK-N-SH and SH-SY5Y whole cell lysates and mouse and rat brain tissue extracts. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclear Produced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-03-15
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