HuD Antibody (H-9): sc-48421

HuD Antibody (H-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects HuD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-HuD antibody (H-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HuD protein, a member of the Elav-like family of RNA-binding proteins, plays a crucial role in neuronal differentiation and maintenance, being expressed in postmitotic neurons. HuD contains three RNA recognition motifs (RRM) that specifically bind to AU-rich elements (ARE) in the 3′-untranslated region of mRNA transcripts. This interaction regulates the stability and degradation of mRNAs, thereby influencing gene expression levels associated with cell growth and differentiation. By binding to ARE sites, HuD inhibits rapid degradation of target mRNAs, leading to increased transcript stability, which is essential for proper neuronal function and development. Dynamic regulation of HuD, including shuttling between nucleus and cytoplasm, ensures accessibility to mRNA targets, making HuD monoclonal antibody (H-9) an invaluable tool for studying molecular mechanisms underlying neuronal biology and related disorders.

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HuD Antibody (H-9) References:

1. Role of HuD and other RNA-binding proteins in neural development and plasticity.  |  Perrone-Bizzozero, N. and Bolognani, F. 2002. J Neurosci Res. 68: 121-6. PMID: 11948657
2. The RNA-binding protein HuD: a regulator of neuronal differentiation, maintenance and plasticity.  |  Deschênes-Furry, J., et al. 2006. Bioessays. 28: 822-33. PMID: 16927307
3. Alternative role of HuD splicing variants in neuronal differentiation.  |  Hayashi, S., et al. 2015. J Neurosci Res. 93: 399-409. PMID: 25332105
4. HuD Binds to and Regulates Circular RNAs Derived From Neuronal Development- and Synaptic Plasticity-Associated Genes.  |  Dell'Orco, M., et al. 2020. Front Genet. 11: 790. PMID: 32849796
5. HuD regulates SOD1 expression during oxidative stress in differentiated neuroblastoma cells and sporadic ALS motor cortex.  |  Dell'Orco, M., et al. 2021. Neurobiol Dis. 148: 105211. PMID: 33271327
6. RNA-Binding Protein HuD Controls Insulin Translation.  |  Lee, EK., et al. 2020. Mol Cell. 80: 1135. PMID: 33338402
7. ALS-related FUS mutations alter axon growth in motoneurons and affect HuD/ELAVL4 and FMRP activity.  |  Garone, MG., et al. 2021. Commun Biol. 4: 1025. PMID: 34471224
8. RNA binding protein HuD promotes autophagy and tumor stress survival by suppressing mTORC1 activity and augmenting ARL6IP1 levels.  |  Bishayee, K., et al. 2022. J Exp Clin Cancer Res. 41: 18. PMID: 35012594
9. Deciphering the Akt1-HuD interaction in HuD-mediated neuronal differentiation.  |  Nishisaka, H., et al. 2024. Biochimie. 221: 20-26. PMID: 38244852
10. HuD (ELAVL4) gain-of-function impairs neuromuscular junctions and induces apoptosis in in vitro and in vivo models of amyotrophic lateral sclerosis.  |  Silvestri, B., et al. 2024. bioRxiv.. PMID: 38464028

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HuD Antibody (H-9) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-HuD Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HuD Antibody (H-9) Specifications

Alternate Product Name: ELAVL4

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: HuD

Epitope Species: human

Product Reactivity/Detection: HuD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for HuD Antibody (H-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HuD antibody is HuD Antibody (E-1): sc-28299.