HuD Antibody (E-1): sc-28299

HuD Antibody (E-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects HuD in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-HuD antibody (E-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HuD, also known as ELAVL4, plays a crucial role in regulating mRNA stability and translation, particularly in postmitotic neurons, where HuD is essential for neuronal differentiation and maintenance. HuD, along with other members of the Elav-like protein family, contains three RNA recognition motifs that specifically bind to AU-rich elements in the 3′-untranslated regions of target mRNAs. This interaction prevents rapid degradation of these mRNAs, thereby enhancing the stability of transcripts critical for neuronal function and development. HuD′s ability to shuttle between the nucleus and cytoplasm, regulated by a nuclear export signal, further underscores HuD′s importance in controlling gene expression in response to cellular signals. HuD monoclonal antibody (E-1) enables researchers to effectively study mRNA regulation dynamics and HuD′s role in various biological processes, including neurogenesis and tumorigenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HuD Antibody (E-1) References:

1. Cytoplasmic localization is required for the mammalian ELAV-like protein HuD to induce neuronal differentiation.  |  Kasashima, K., et al. 1999. Genes Cells. 4: 667-83. PMID: 10620013
2. Role of HuD in nervous system function and pathology.  |  Perrone-Bizzozero, N. and Bird, CW. 2013. Front Biosci (Schol Ed). 5: 554-63. PMID: 23277068
3. Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction.  |  Bronicki, LM. and Jasmin, BJ. 2013. RNA. 19: 1019-37. PMID: 23861535
4. RNA-Binding Protein HuD as a Versatile Factor in Neuronal and Non-Neuronal Systems.  |  Jung, M. and Lee, EK. 2021. Biology (Basel). 10: PMID: 33922479
5. Emerging Roles for the RNA-Binding Protein HuD (ELAVL4) in Nervous System Diseases.  |  Silvestri, B., et al. 2022. Int J Mol Sci. 23: PMID: 36498933
6. Differential activity of ELAV-like RNA-binding proteins in human neuroblastoma.  |  Chagnovich, D., et al. 1996. J Biol Chem. 271: 33587-91. PMID: 8969226
7. The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mRNA.  |  Chung, S., et al. 1997. J Biol Chem. 272: 6593-8. PMID: 9045688
8. Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.  |  Myer, VE., et al. 1997. EMBO J. 16: 2130-9. PMID: 9155038
9. Differential expression of the neuroendocrine genes Hel-N1 and HuD in small-cell lung carcinoma: evidence for down-regulation of HuD in the variant phenotype.  |  King, PH. 1997. Int J Cancer. 74: 378-82. PMID: 9291425
10. Sequential expression and role of Hu RNA-binding proteins during neurogenesis.  |  Wakamatsu, Y. and Weston, JA. 1997. Development. 124: 3449-60. PMID: 9310339
11. RNA stabilization by the AU-rich element binding protein, HuR, an ELAV protein.  |  Peng, SS., et al. 1998. EMBO J. 17: 3461-70. PMID: 9628881
12. Neuron-specific hel-N1 and HuD as novel molecular markers of neuroblastoma: a correlation of HuD messenger RNA levels with favorable prognostic features.  |  Ball, NS. and King, PH. 1997. Clin Cancer Res. 3: 1859-65. PMID: 9815574

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HuD Antibody (E-1) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-HuD Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HuD Antibody (E-1) Specifications

Alternate Product Name: ELAVL4

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: HuD

Epitope Species: human

Product Reactivity/Detection: HuD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for HuD Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HuD antibody is HuD Antibody (H-9): sc-48421.