HuD Antibody (E-1) recommended for detection of HuD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HuD Antibody (E-1): sc-28299

HuD Antibody (E-1) is rated 4.2 out of 5 by 5.
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Datasheets

    • HuD Antibody (E-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 of HuD of human origin
    • Anti-HuD Antibody (E-1) is recommended for detection of HuD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HuD Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HuD (E-1): sc-28299.
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Is HuD (E-1): sc-28299 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using HuD (H-9): sc-48421, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/hud-antibody-h-9)
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 1 out of 5 by from Antibody not specific Be careful with this antibody. It has been produced using almost the full ELAVL4 protein without taking into account the high sequence homology between ELAVL4 and ELAVL2 (91%), and with ELAVL3 (85%). I have observed the same specificy for ELAVL4, ELAVL2 and ELAVL4 using this antibody I'm really disappointed!!
Date published: 2018-10-24
Rated 5 out of 5 by from Excellent HuD antibody for WB The E-1 clone works well in WB for mouse, rat and human.
Date published: 2016-12-22
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human cerebellum tissue. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Good publishable data in WB in human Medulloblastoma Good publishable data in WB in human Medulloblastoma Cells. -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by from Robust signal in WB in SK Robust signal in WB in SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-02-17
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