HuC Antibody (D-6): sc-515624

HuC Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects HuC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-HuC antibody (D-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HuC protein, part of the Elav-like family of RNA-binding proteins, plays a crucial role in neuronal differentiation and maintenance, being expressed in postmitotic neurons. These proteins, including HuB, HuC, and HuD, are characterized by three RNA recognition motifs, which enable specific binding to AU-rich elements in the 3′-untranslated regions of mRNA transcripts. This interaction prevents rapid degradation of mRNA, thereby stabilizing transcripts essential for regulating gene expression involved in cell growth and differentiation. HuC′s ability to shuttle between the nucleus and cytoplasm, facilitated by a nuclear export signal, ensures accessibility to target mRNA, ultimately influencing neuronal function and development.

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HuC Antibody (D-6) References:

1. Cytoplasmic localization is required for the mammalian ELAV-like protein HuD to induce neuronal differentiation.  |  Kasashima, K., et al. 1999. Genes Cells. 4: 667-83. PMID: 10620013
2. Differential activity of ELAV-like RNA-binding proteins in human neuroblastoma.  |  Chagnovich, D., et al. 1996. J Biol Chem. 271: 33587-91. PMID: 8969226
3. The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mRNA.  |  Chung, S., et al. 1997. J Biol Chem. 272: 6593-8. PMID: 9045688
4. Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.  |  Myer, VE., et al. 1997. EMBO J. 16: 2130-9. PMID: 9155038
5. Differential expression of the neuroendocrine genes Hel-N1 and HuD in small-cell lung carcinoma: evidence for down-regulation of HuD in the variant phenotype.  |  King, PH. 1997. Int J Cancer. 74: 378-82. PMID: 9291425
6. Sequential expression and role of Hu RNA-binding proteins during neurogenesis.  |  Wakamatsu, Y. and Weston, JA. 1997. Development. 124: 3449-60. PMID: 9310339
7. RNA stabilization by the AU-rich element binding protein, HuR, an ELAV protein.  |  Peng, SS., et al. 1998. EMBO J. 17: 3461-70. PMID: 9628881
8. Neuron-specific hel-N1 and HuD as novel molecular markers of neuroblastoma: a correlation of HuD messenger RNA levels with favorable prognostic features.  |  Ball, NS. and King, PH. 1997. Clin Cancer Res. 3: 1859-65. PMID: 9815574

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HuC Antibody (D-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HuC Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HuC Antibody (D-6) Specifications

Alternate Product Name: HuC

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 265-287

Epitope Target: HuC

Epitope Species: human

Product Reactivity/Detection: HuC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HuC Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin