Date published: 2026-3-17

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HSP70 Antibody (F-3): sc-373867

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Datasheets
  • HSP70 Antibody (F-3) is a mouse monoclonal IgG2b κ, cited in 20 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 565-601 near the C-terminus of HSP 70 of human origin
  • Anti-HSP70 Antibody (F-3) is recommended for detection of HSP 70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
  • Anti-HSP70 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HSP70 Antibody (F-3) for WB and IHC(P) applications. These reagents are now offered in bundles with HSP70 Antibody (F-3) (see ordering information below).
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HSP 70 Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects HSP 70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HSP 70 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSP 70 plays a crucial role as a molecular chaperone, facilitating proper folding of nascent polypeptide chains and preventing misfolded protein aggregation, which maintains cellular homeostasis and function. HSP 70 expression increases significantly under stress conditions, such as heat shock, protecting cells from damage. HSP 70 primarily resides in the cytosol and nucleus of mammalian cells, assisting in polypeptide translocation across cellular membranes and into the nucleus, ensuring proteins reach correct destinations and maintain functional conformations. HSP 70′s response to cellular stressors showcases its importance in cellular protection and recovery, making HSP 70 essential in various physiological and pathological processes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

HSP70 Antibody (F-3)

sc-373867
200 µg/ml
$322.00

HSP70 Antibody (F-3): m-IgG Fc BP-HRP Bundle

sc-529593
200 µg Ab; 10 µg BP
$361.00

HSP70 Antibody (F-3): m-IgGκ BP-HRP Bundle

sc-522537
200 µg Ab, 40 µg BP
$361.00

HSP70 Antibody (F-3) AC

sc-373867 AC
500 µg/ml, 25% agarose
$424.00

HSP70 Antibody (F-3) HRP

sc-373867 HRP
200 µg/ml
$322.00

HSP70 Antibody (F-3) FITC

sc-373867 FITC
200 µg/ml
$336.00

HSP70 Antibody (F-3) PE

sc-373867 PE
200 µg/ml
$349.00

HSP70 Antibody (F-3) Alexa Fluor® 488

sc-373867 AF488
200 µg/ml
$364.00

HSP70 Antibody (F-3) Alexa Fluor® 546

sc-373867 AF546
200 µg/ml
$364.00

HSP70 Antibody (F-3) Alexa Fluor® 594

sc-373867 AF594
200 µg/ml
$364.00

HSP70 Antibody (F-3) Alexa Fluor® 647

sc-373867 AF647
200 µg/ml
$364.00

HSP70 Antibody (F-3) Alexa Fluor® 680

sc-373867 AF680
200 µg/ml
$364.00

HSP70 Antibody (F-3) Alexa Fluor® 790

sc-373867 AF790
200 µg/ml
$364.00

HSP70 (F-3) Neutralizing Peptide

sc-373867 P
100 µg/0.5 ml
$69.00

can i use this HSP70 anti body for western bloting in manila clam sample

Asked by: jahan kifat
Thank you for your question. Anti-HSP 70 Antibody (F-3) is only recommended for detection of HSP 70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, as well as canine, bovine and porcine samples.
Answered by: Tech Support Europe
Date published: 2021-10-18

I would like to use HSP 70 (F-3): sc-373867 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-373867 is not available directly conjugated, but we have an alternative HSP 70 antibody (sc-24) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-17

What is the recommended positive control for HSP 70 (F-3): sc-373867?

Asked by: Trav11
Thank you for your question. For HSP 70 (F-3): sc-373867, we have several recommended positive controls, which include: HeLa Whole Cell Lysate (sc-2200), NIH/3T3 Whole Cell Lysate (sc-2210), HUV-EC-C Whole Cell Lysate, as well as a few others. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-13
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Rated 5 out of 5 by from Well for WB,but careful for Co-ipThis antibody gives a strong and clear bands in K562, but if you perform Co-ip, you should increase the total protein amount from cell lysates.
Date published: 2020-08-06
Rated 1 out of 5 by from Bad AntibodyThis antibody has obvious unspecial band at 55 kD , and the IP result is not very well using this one .
Date published: 2018-04-30
Rated 4 out of 5 by from goodWestern blot, AsPC-1 cell lysate, loading 5ug, antibody 1:500 dilution, band at right size, but not very strong.
Date published: 2018-02-09
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from great antibody for westernIt's a great antibody for western and the band at 70 kD is very clear.
Date published: 2017-09-18
Rated 5 out of 5 by from Great WB and IHCOutstanding WB data; strong, specific signal; also great IHC staining
Date published: 2017-01-30
Rated 5 out of 5 by from Good HSP 70 detectionGreat, published antibody for IHC, also recommended for WB, IP, IF, and ELISA. Strong signal for multiple tissue types and cell lines.
Date published: 2017-01-24
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody, good specificity and strong signal strength in HeLa and NIH/3T3. -SCBT QC
Date published: 2015-04-08
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HSP70 Antibody (F-3) is rated 4.3 out of 5 by 9.
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