HSP70 Antibody (3A3): sc-32239
- HSP70 Antibody (3A3) is a mouse monoclonal IgG1 κ HSP70 antibody, cited in 208 publications, provided at 200 µg/ml
- raised against recombinant human HSP 70
- HSP70 Antibody (3A3) is recommended for detection of HSP 70 and HSC 70 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-HSP70 Antibody (3A3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HSP70 Antibody (3A3) for WB and IHC(P) applications. These reagents are now offered in bundles with HSP70 Antibody (3A3) (see ordering information below).
HSP 70 Antibody (3A3) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 70 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-HSP 70 antibody (3A3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSP 70 (3A3) antibody recognizes heat shock protein A (Hsp70), which plays a crucial role as a molecular chaperone, facilitating proper folding of nascent polypeptide chains and preventing aggregation of misfolded proteins - vital for maintaining cellular homeostasis, especially under stress conditions. HSP 70 protein is predominantly located in the cytosol and nucleus of mammalian cells, where HSP 70 assists in translocation of polypeptides across cellular membranes and into the nucleus. HSP 70 expression significantly increases in response to heat stress, highlighting HSP 70′s importance in cellular protection and recovery during stressful conditions. HSP 70 family, including HSC 70, GRP 75, and GRP 78, collectively contributes to various cellular processes, including assembly of multi-protein complexes and import of proteins into organelles, underscoring HSP 70′s essential role in cellular function and survival.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HSP70 Antibody (3A3) References:
- Prevention of protein denaturation under heat stress by the chaperonin Hsp60. | Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
- Effects of low culture temperature on the induction of hsp70 mRNA and the accumulation of hsp70 and hsp105 in mouse FM3A cells. | Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
- Cloning of rat grp75, an hsp70-family member, and its expression in normal and ischemic brain. | Massa, SM., et al. 1995. J Neurosci Res. 40: 807-19. PMID: 7629893
- Protein-mediated protein maturation in eukaryotes. | Haas, IG. 1995. FEBS Lett. 369: 72-5. PMID: 7641888
- Can Hsp70 proteins act as force-generating motors? | Glick, BS. 1995. Cell. 80: 11-4. PMID: 7813006
- Cloning and subcellular localization of human mitochondrial hsp70. | Bhattacharyya, T., et al. 1995. J Biol Chem. 270: 1705-10. PMID: 7829505
- Autophosphorylation of grp94 (endoplasmin). | Csermely, P., et al. 1995. J Biol Chem. 270: 6381-8. PMID: 7890776
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP70 Antibody (3A3) | sc-32239 | 200 µg/ml | $322.00 | |||
HSP70 Antibody (3A3): m-IgG Fc BP-HRP Bundle | sc-528445 | 200 µg Ab; 10 µg BP | $361.00 | |||
HSP70 Antibody (3A3): m-IgGκ BP-HRP Bundle | sc-520824 | 200 µg Ab, 40 µg BP | $361.00 | |||
HSP70 Antibody (3A3): m-IgG1 BP-HRP Bundle | sc-542935 | 200 µg Ab; 20 µg BP | $361.00 | |||
HSP70 Antibody (3A3) AC | sc-32239 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HSP70 Antibody (3A3) HRP | sc-32239 HRP | 200 µg/ml | $322.00 | |||
HSP70 Antibody (3A3) FITC | sc-32239 FITC | 200 µg/ml | $336.00 | |||
HSP70 Antibody (3A3) PE | sc-32239 PE | 200 µg/ml | $349.00 | |||
HSP70 Antibody (3A3) Alexa Fluor® 488 | sc-32239 AF488 | 200 µg/ml | $364.00 | |||
HSP70 Antibody (3A3) Alexa Fluor® 546 | sc-32239 AF546 | 200 µg/ml | $364.00 | |||
HSP70 Antibody (3A3) Alexa Fluor® 594 | sc-32239 AF594 | 200 µg/ml | $364.00 | |||
HSP70 Antibody (3A3) Alexa Fluor® 647 | sc-32239 AF647 | 200 µg/ml | $364.00 | |||
HSP70 Antibody (3A3) Alexa Fluor® 680 | sc-32239 AF680 | 200 µg/ml | $364.00 | |||
HSP70 Antibody (3A3) Alexa Fluor® 790 | sc-32239 AF790 | 200 µg/ml | $364.00 |