HSP 40 Antibody (B-3) recommended for detection of HSP 40 subfamily B member 1 of mouse, rat and human origin by WB, IP, IF and ELISA

HSP 40 Antibody (B-3): sc-398766

HSP 40 Antibody (B-3) is rated 4.9 out of 5 by 7.
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Datasheets

    • Anti-HSP 40 Antibody (B-3) is a mouse monoclonal IgG1 κ HSP 40 antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 241-340 mapping at the C-terminus of HSP 40 protein 1 of human origin
    • Anti-HSP 40 Antibody (B-3) is recommended for detection of HSP 40 subfamily B member 1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and bovine
    • Anti-HSP 40 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HSP 40 (B-3): sc-398766.

What is the recommended starting dilution for Western blot for sc-398766: HSP 40 (B-3) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Very good antibody for WB Very good and specific band detection at diluition 1:1000 in western blotting of human bronchial cell lines 16-HBE
Date published: 2018-10-05
Rated 5 out of 5 by from Great antibody! 1:500 diluted for wb of rat protein,the band is strong and special
Date published: 2018-01-31
Rated 5 out of 5 by from Works well for WB works well for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from great antibody for western blot I recently used it for western blot at 1/1000 dilution, and it gives very good bands.
Date published: 2017-08-29
Rated 5 out of 5 by from Great for WB The HSP 40 Antibody (B-3): sc-398766 works very well for Western blot applications, showing clear band with minimal background.
Date published: 2017-01-18
Rated 4 out of 5 by from Potent and quite specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~40 kDa target protein in an A431/HepG2/MCF7 cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested. A ~25 kDa strongly cross-reactive protein was detectable in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Great antibody for Western Great antibody for Western, strong band detected in K-562 and Jurkat. -SCBT QC
Date published: 2015-04-06
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