HSP 40 Antibody (B-3): sc-398766

HSP 40 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 40 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HSP 40 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Heat shock protein 40 (HSP 40), also known as DnaJ, plays a crucial role in cellular stress responses by functioning as a molecular chaperone. HSP 40 interacts with HSP 70 through its J-domain, enhancing the ATPase activity of HSP 70, which is vital for protein folding and preventing aggregation of misfolded proteins. HSP 40 is predominantly located in the cytoplasm but translocates to the nucleus and nucleoli in response to heat shock, highlighting its importance in cellular adaptation to stress. This dynamic localization is essential for the regulation of protein homeostasis and the maintenance of cellular function under stress conditions. With five known family members, HSP 40 is integral to the assembly and disassembly of protein complexes, ensuring proper protein maturation and function.

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HSP 40 Antibody (B-3) References:

1. Heat shock protein (Hsp) 40 mutants inhibit Hsp70 in mammalian cells.  |  Michels, AA., et al. 1999. J Biol Chem. 274: 36757-63. PMID: 10593983
2. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature.  |  Ohtsuka, K. and Hata, M. 2000. Cell Stress Chaperones. 5: 98-112. PMID: 11147971
3. Specificity of class II Hsp40 Sis1 in maintenance of yeast prion [RNQ+].  |  Lopez, N., et al. 2003. Mol Biol Cell. 14: 1172-81. PMID: 12631732
4. Expression of heat shock protein (Hsp) 70 and Hsp 40 in colorectal cancer.  |  Kanazawa, Y., et al. 2003. Med Oncol. 20: 157-64. PMID: 12835518
5. Novel role of HSP40/DNAJ in the regulation of HIV-1 replication.  |  Urano, E., et al. 2013. J Acquir Immune Defic Syndr. 64: 154-62. PMID: 24047968
6. The endoplasmic reticulum HSP40 co-chaperone ERdj3/DNAJB11 assembles and functions as a tetramer.  |  Chen, KC., et al. 2017. EMBO J. 36: 2296-2309. PMID: 28655754
7. Targeting the Hsp40/Hsp70 Chaperone Axis as a Novel Strategy to Treat Castration-Resistant Prostate Cancer.  |  Moses, MA., et al. 2018. Cancer Res. 78: 4022-4035. PMID: 29764864
8. Cotranslocation and colocalization of hsp40 (DnaJ) with hsp70 (DnaK) in mammalian cells.  |  Yamane, M., et al. 1995. Cell Struct Funct. 20: 157-66. PMID: 7641298
9. Heat-shock protein 40, a novel predictor of thermotolerance in murine cells.  |  Kaneko, R., et al. 1995. Radiat Res. 142: 91-7. PMID: 7899564
10. Effect of ATP on the release of hsp 70 and hsp 40 from the nucleus in heat-shocked HeLa cells.  |  Ohtsuka, K., et al. 1993. Exp Cell Res. 209: 357-66. PMID: 8262154
11. Isolation of a new member of DnaJ-like heat shock protein 40 (Hsp40) from human liver.  |  Hoe, KL., et al. 1998. Biochim Biophys Acta. 1383: 4-8. PMID: 9546042
12. Presence of molecular chaperones, heat shock cognate (Hsc) 70 and heat shock proteins (Hsp) 40, in the postsynaptic structures of rat brain.  |  Suzuki, T., et al. 1999. Brain Res. 816: 99-110. PMID: 9878698

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 40 Antibody (B-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 40 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 40 Antibody (B-3) Specifications

Alternate Product Name: HSP 40

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-340

Epitope Target: HSP 40 protein 1

Epitope Species: human

Product Reactivity/Detection: HSP 40 subfamily B member 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for HSP 40 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin