hSNF2H Antibody (D-10): sc-365727

hSNF2H (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects hSNF2H protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). anti-hSNF2H antibody (D-10) is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2a) and Brg-1 (also designated SNF2b) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila Isw1, a BRM related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

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hSNF2H Antibody (D-10) References:

1. Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.  |  Phelan, ML., et al. 1999. Mol Cell. 3: 247-53. PMID: 10078207
2. DNMT3B interacts with hSNF2H chromatin remodeling enzyme, HDACs 1 and 2, and components of the histone methylation system.  |  Geiman, TM., et al. 2004. Biochem Biophys Res Commun. 318: 544-55. PMID: 15120635
3. The roles of human sucrose nonfermenting protein 2 homologue in the tumor-promoting functions of Rsf-1.  |  Sheu, JJ., et al. 2008. Cancer Res. 68: 4050-7. PMID: 18519663
4. Functional analysis of 11q13.5 amplicon identifies Rsf-1 (HBXAP) as a gene involved in paclitaxel resistance in ovarian cancer.  |  Choi, JH., et al. 2009. Cancer Res. 69: 1407-15. PMID: 19190325
5. A novel t(4;22)(q31;q12) produces an EWSR1-SMARCA5 fusion in extraskeletal Ewing sarcoma/primitive neuroectodermal tumor.  |  Sumegi, J., et al. 2011. Mod Pathol. 24: 333-42. PMID: 21113140
6. Williams syndrome is an epigenome-regulator disease.  |  Kitagawa, H., et al. 2011. Endocr J. 58: 77-85. PMID: 21242649
7. Overexpression of hSNF2H in glioma promotes cell proliferation, invasion, and chemoresistance through its interaction with Rsf-1.  |  Zhao, XC., et al. 2016. Tumour Biol. 37: 7203-12. PMID: 26666816
8. A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor.  |  Muchardt, C. and Yaniv, M. 1993. EMBO J. 12: 4279-90. PMID: 8223438
9. BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.  |  Khavari, PA., et al. 1993. Nature. 366: 170-4. PMID: 8232556
10. ISWI, a member of the SWI2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor.  |  Tsukiyama, T., et al. 1995. Cell. 83: 1021-6. PMID: 8521502
11. Nucleosome disruption by human SWI/SNF is maintained in the absence of continued ATP hydrolysis.  |  Imbalzano, AN., et al. 1996. J Biol Chem. 271: 20726-33. PMID: 8702824
12. Cloning and mapping of SMARCA5 encoding hSNF2H, a novel human homologue of Drosophila ISWI.  |  Aihara, T., et al. 1998. Cytogenet Cell Genet. 81: 191-3. PMID: 9730600

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hSNF2H Antibody (D-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-hSNF2H Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hSNF2H Antibody (D-10) Specifications

Alternate Product Name: hSNF2H

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 753-1052

Epitope Target: hSNF2H

Epitope Species: human

Product Reactivity/Detection: hSNF2H

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for hSNF2H Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent