HSF4 Antibody (A-12) recommended for detection of HSF4 isoforms a and b of mouse, rat and human origin by WB, IP, IF and ELISA

HSF4 Antibody (A-12): sc-398645

HSF4 Antibody (A-12) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-HSF4 Antibody (A-12) is a mouse monoclonal IgG1 (kappa light chain) HSF4 antibody provided at 200 µg/ml
    • raised against amino acids 120-244 mapping within an internal region of HSF4 of human origin
    • Anti-HSF4 Antibody (A-12) is recommended for detection of HSF4 isoforms a and b of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-HSF4 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HSF4 (A-12): sc-398645.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with HSF4 (A-12): sc-398645 monoclonal antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Highly potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~55 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Very good Western blot data of HSF4 expression Very good Western blot data of HSF4 expression in HEK293 whole cell lysate and mouse brain tissue extract . -SCBT QC
Date published: 2013-04-06
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