HSF4 Antibody (A-12): sc-398645

HSF4 Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSF4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HSF4 Antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSF4 plays a crucial role in cellular response to stress, particularly in regulating heat shock proteins, which are essential for protein folding and protection against cellular damage. HSF4 is expressed as two splice variants, HSF4α and HSF4β, with distinct functions; HSF4α lacks a DNA-binding domain and inhibits HSF1-mediated transcription, while HSF4β contains a DNA-binding domain and participates in nuclear body formation during heat shock. This functional diversity maintains cellular homeostasis and ensures proper stress responses, particularly in tissues such as heart, brain, and skeletal muscle where HSF4 shows prominent expression. HSF4′s ability to modulate other heat shock transcription factors highlights anti-HSF4 antibody (A-12) as a valuable tool for studying cellular stress responses and related pathways.

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HSF4 Antibody (A-12) References:

1. Heat shock factor-4 (HSF-4a) is a repressor of HSF-1 mediated transcription.  |  Zhang, Y., et al. 2001. J Cell Biochem. 82: 692-703. PMID: 11500947
2. HSF4 promotes G1/S arrest in human lens epithelial cells by stabilizing p53.  |  Huang, M., et al. 2015. Biochim Biophys Acta. 1853: 1808-17. PMID: 25940838
3. Mutation update of transcription factor genes FOXE3, HSF4, MAF, and PITX3 causing cataracts and other developmental ocular defects.  |  Anand, D., et al. 2018. Hum Mutat. 39: 471-494. PMID: 29314435
4. HSF4 transcriptional regulates HMOX-1 expression in HLECs.  |  Liao, S., et al. 2018. Gene. 655: 30-34. PMID: 29454088
5. Transcriptional activation of heat-shock genes in eukaryotes.  |  Tanguay, RM. 1988. Biochem Cell Biol. 66: 584-93. PMID: 3048332
6. HSF4 promotes tumor progression of colorectal cancer by transactivating c-MET.  |  Zhang, W., et al. 2023. Mol Cell Biochem. 478: 1141-1150. PMID: 36229759
7. HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation.  |  Zhang, J., et al. 2023. Invest Ophthalmol Vis Sci. 64: 5. PMID: 37266953
8. HSF4/COIL complex-dependent R-loop mediates ultraviolet-induced inflammatory skin injury.  |  Feng, YQ., et al. 2023. Clin Transl Med. 13: e1336. PMID: 37461263
9. Estrogen dependent expression of heat shock transcription factor: implications for uterine synthesis of heat shock proteins.  |  Yang, X., et al. 1995. J Steroid Biochem Mol Biol. 52: 415-9. PMID: 7748806
10. Determination of heat-shock transcription factor 2 stoichiometry at looped DNA complexes using scanning force microscopy.  |  Wyman, C., et al. 1995. EMBO J. 14: 117-23. PMID: 7828583
11. HSF4, a new member of the human heat shock factor family which lacks properties of a transcriptional activator.  |  Nakai, A., et al. 1997. Mol Cell Biol. 17: 469-81. PMID: 8972228
12. Glycogen synthase kinase 3beta and extracellular signal-regulated kinase inactivate heat shock transcription factor 1 by facilitating the disappearance of transcriptionally active granules after heat shock.  |  He, B., et al. 1998. Mol Cell Biol. 18: 6624-33. PMID: 9774677

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSF4 Antibody (A-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HSF4 Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSF4 Antibody (A-12) Specifications

Alternate Product Name: HSF4

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 120-244

Epitope Target: HSF4

Epitope Species: human

Product Reactivity/Detection: HSF4 isoforms a and b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HSF4 Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin