HSF2 Antibody (G-11): sc-74529

HSF2 Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSF2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HSF2 antibody (G-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSF2 plays a crucial role in cellular response to stress, particularly in regulating heat shock genes, which are essential for protecting cells from damage caused by elevated temperatures and other stressors. HSF2 is primarily located in the nucleus, where HSF2 functions as a transcription factor that binds to heat shock elements in promoter regions of target genes. This nuclear localization enables HSF2 to effectively regulate gene expression in response to stress, ensuring appropriate protective mechanisms are activated. HSF2 interacts with HSF1, another heat shock transcription factor, and both factors can form trimers to bind DNA, highlighting their collaborative role in stress response and cellular homeostasis. HSF2′s ability to be upregulated by estrogen further underscores HSF2′s importance in developmental processes and differentiation, making HSF2 (G-11) monoclonal antibody an invaluable tool for researchers studying stress response mechanisms and related pathways in various biological contexts.

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HSF2 Antibody (G-11) References:

1. MEL-18 interacts with HSF2 and the SUMO E2 UBC9 to inhibit HSF2 sumoylation.  |  Zhang, J., et al. 2008. J Biol Chem. 283: 7464-9. PMID: 18211895
2. PRC1 associates with the hsp70i promoter and interacts with HSF2 during mitosis.  |  Murphy, LA., et al. 2008. Exp Cell Res. 314: 2224-30. PMID: 18570919
3. The proteasome inhibitor bortezomib is a potent inducer of zinc finger AN1-type domain 2a gene expression: role of heat shock factor 1 (HSF1)-heat shock factor 2 (HSF2) heterocomplexes.  |  Rossi, A., et al. 2014. J Biol Chem. 289: 12705-15. PMID: 24619424
4. Transcriptional activation of heat-shock genes in eukaryotes.  |  Tanguay, RM. 1988. Biochem Cell Biol. 66: 584-93. PMID: 3048332
5. The proteostasis guardian HSF1 directs the transcription of its paralog and interactor HSF2 during proteasome dysfunction.  |  Santopolo, S., et al. 2021. Cell Mol Life Sci. 78: 1113-1129. PMID: 32607595
6. Polymorphisms in the HSF2, LRRC6, MEIG1 and PTIP genes correlate with sperm motility in idiopathic infertility.  |  Gupta, N., et al. 2022. Andrologia. 54: e14517. PMID: 35768906
7. Estrogen dependent expression of heat shock transcription factor: implications for uterine synthesis of heat shock proteins.  |  Yang, X., et al. 1995. J Steroid Biochem Mol Biol. 52: 415-9. PMID: 7748806
8. Determination of heat-shock transcription factor 2 stoichiometry at looped DNA complexes using scanning force microscopy.  |  Wyman, C., et al. 1995. EMBO J. 14: 117-23. PMID: 7828583
9. Function and regulation of heat shock factor 2 during mouse embryogenesis.  |  Rallu, M., et al. 1997. Proc Natl Acad Sci U S A. 94: 2392-7. PMID: 9122205
10. Heat shock response and protein degradation: regulation of HSF2 by the ubiquitin-proteasome pathway.  |  Mathew, A., et al. 1998. Mol Cell Biol. 18: 5091-8. PMID: 9710593
11. Proteasome inhibition leads to the activation of all members of the heat-shock-factor family.  |  Kawazoe, Y., et al. 1998. Eur J Biochem. 255: 356-62. PMID: 9716376
12. Glycogen synthase kinase 3beta and extracellular signal-regulated kinase inactivate heat shock transcription factor 1 by facilitating the disappearance of transcriptionally active granules after heat shock.  |  He, B., et al. 1998. Mol Cell Biol. 18: 6624-33. PMID: 9774677

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSF2 Antibody (G-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HSF2 Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSF2 Antibody (G-11) Specifications

Alternate Product Name: HSF2

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 237-536

Epitope Target: HSF2

Epitope Species: human

Product Reactivity/Detection: HSF2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HSF2 Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent