HSF1 Antibody (10H8): sc-13516
- HSF1 Antibody (10H8) is a rat monoclonal IgG1, cited in 18 publications, provided at 200 µg/ml
- raised against recombinant HSF1 of mouse origin, with epitope mapping to amino acids 378-395
- Anti-HSF1 Antibody (10H8) is recommended for detection of HSF1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-HSF1 Antibody (10H8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-13516 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HSF1 Antibody (10H8). This work is in progress.
QUICK LINKS
HSF1 Antibody (10H8) is a rat monoclonal IgG1 antibody that detects HSF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). HSF1 (10H8) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. HSF1, or heat shock factor 1, plays a crucial role in the cellular response to stress by regulating the expression of heat shock proteins, which are essential for protein folding and protection against cellular damage. HSF1 is primarily located in the cytoplasm under normal conditions, where HSF1 exists as a monomer; however, upon stress, HSF1 undergoes a conformational change, allowing trimerization and translocation to the nucleus. This translocation enables HSF1 to bind to heat shock elements in the promoter regions of target genes, thereby initiating the transcription of heat shock proteins that help maintain cellular homeostasis during stressful conditions. HSF1 regulation is influenced by post-translational modifications, such as phosphorylation, which can either activate or repress transcriptional activity, highlighting the importance of precise control over HSF1 function in cellular stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSF1 Antibody (10H8) | sc-13516 | 200 µg/ml | $322.00 | |||
HSF1 Antibody (10H8) X | sc-13516 X | 200 µg/0.1 ml | $322.00 | |||
HSF1 Antibody (10H8) AC | sc-13516 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HSF1 Antibody (10H8) HRP | sc-13516 HRP | 200 µg/ml | $322.00 | |||
HSF1 Antibody (10H8) FITC | sc-13516 FITC | 200 µg/ml | $336.00 | |||
HSF1 Antibody (10H8) PE | sc-13516 PE | 200 µg/ml | $349.00 | |||
HSF1 Antibody (10H8) Alexa Fluor® 488 | sc-13516 AF488 | 200 µg/ml | $364.00 | |||
HSF1 Antibody (10H8) Alexa Fluor® 546 | sc-13516 AF546 | 200 µg/ml | $364.00 | |||
HSF1 Antibody (10H8) Alexa Fluor® 594 | sc-13516 AF594 | 200 µg/ml | $364.00 | |||
HSF1 Antibody (10H8) Alexa Fluor® 647 | sc-13516 AF647 | 200 µg/ml | $364.00 | |||
HSF1 Antibody (10H8) Alexa Fluor® 680 | sc-13516 AF680 | 200 µg/ml | $364.00 | |||
HSF1 Antibody (10H8) Alexa Fluor® 790 | sc-13516 AF790 | 200 µg/ml | $364.00 |