HRPAP20 Antibody (G-10): sc-166928
- HRPAP20 Antibody (G-10) is a mouse monoclonal IgG2b κ HRPAP20 antibody provided at 200 µg/ml
- raised against amino acids 1-175 representing full length HRPAP20 of human origin
- HRPAP20 Antibody (G-10) is recommended for detection of HRPAP20 of human origin by WB, IP, IF and ELISA
- Anti-HRPAP20 Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HRPAP20 Antibody (G-10) for WB applications. These reagents are now offered in bundles with HRPAP20 Antibody (G-10) (see ordering information below).
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HRPAP20 Antibody (G-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects HRPAP20 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HRPAP20 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HRPAP20 protein, also known as hormone-regulated proliferation-associated protein 20, consists of 175 amino acids and is encoded by the human gene NDUFAF4. HRPAP20 plays a crucial role in cellular proliferation and apoptosis regulation, making HRPAP20 significant in cancer biology. HRPAP20 enhances cell proliferation and suppresses apoptosis in tumor cell lines, characteristics often linked to malignant progression. Elevated levels of HRPAP20 have been observed in invasive breast cancer cell lines and human breast tumor specimens, indicating HRPAP20′s potential role in increasing malignant cell invasion. Transfection of cell lines such as MCF-7 and MDA-MB-231 with HRPAP20 leads to increased secretion of matrix metalloproteinase-9 (MMP-9), an enzyme associated with tumor invasion and metastasis. Knockdown of HRPAP20 using small interfering RNA results in reduced invasion and inhibition of MMP-9 secretion, highlighting HRPAP20′s importance in cancer progression and potential as a therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HRPAP20 Antibody (G-10) References:
- Primary breast myoepithelial cells exert an invasion-suppressor effect on breast cancer cells via paracrine down-regulation of MMP expression in fibroblasts and tumour cells. | Jones, JL., et al. 2003. J Pathol. 201: 562-72. PMID: 14648659
- Identification of HRPAP20: a novel phosphoprotein that enhances growth and survival in hormone-responsive tumor cells. | Karp, CM., et al. 2004. Cancer Res. 64: 1016-25. PMID: 14871833
- Expression of membrane type-1 matrix metalloproteinase, MT1-MMP in human breast cancer and its impact on invasiveness of breast cancer cells. | Jiang, WG., et al. 2006. Int J Mol Med. 17: 583-90. PMID: 16525713
- Inhibition of cyclooxygenase-2 decreases breast cancer cell motility, invasion and matrix metalloproteinase expression. | Larkins, TL., et al. 2006. BMC Cancer. 6: 181. PMID: 16831226
- A splice variant of CD99 increases motility and MMP-9 expression of human breast cancer cells through the AKT-, ERK-, and JNK-dependent AP-1 activation signaling pathways. | Byun, HJ., et al. 2006. J Biol Chem. 281: 34833-47. PMID: 16984917
- HRPAP20: a novel calmodulin-binding protein that increases breast cancer cell invasion. | Karp, CM., et al. 2007. Oncogene. 26: 1780-8. PMID: 17001319
- Oligonucleotide microarray analysis of estrogen receptor alpha-positive postmenopausal breast carcinomas: identification of HRPAP20 and TIMELESS as outstanding candidate markers to predict the response to tamoxifen. | Tozlu-Kara, S., et al. 2007. J Mol Endocrinol. 39: 305-18. PMID: 17909269
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HRPAP20 Antibody (G-10) | sc-166928 | 200 µg/ml | $322.00 | |||
HRPAP20 Antibody (G-10): m-IgGκ BP-HRP Bundle | sc-521802 | 200 µg Ab, 40 µg BP | $361.00 | |||
HRPAP20 Antibody (G-10): m-IgG2b BP-HRP Bundle | sc-548970 | 200 µg Ab; 10 µg BP | $361.00 | |||
HRPAP20 Antibody (G-10) AC | sc-166928 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HRPAP20 Antibody (G-10) HRP | sc-166928 HRP | 200 µg/ml | $322.00 | |||
HRPAP20 Antibody (G-10) FITC | sc-166928 FITC | 200 µg/ml | $336.00 | |||
HRPAP20 Antibody (G-10) PE | sc-166928 PE | 200 µg/ml | $349.00 | |||
HRPAP20 Antibody (G-10) Alexa Fluor® 488 | sc-166928 AF488 | 200 µg/ml | $364.00 | |||
HRPAP20 Antibody (G-10) Alexa Fluor® 546 | sc-166928 AF546 | 200 µg/ml | $364.00 | |||
HRPAP20 Antibody (G-10) Alexa Fluor® 594 | sc-166928 AF594 | 200 µg/ml | $364.00 | |||
HRPAP20 Antibody (G-10) Alexa Fluor® 647 | sc-166928 AF647 | 200 µg/ml | $364.00 | |||
HRPAP20 Antibody (G-10) Alexa Fluor® 680 | sc-166928 AF680 | 200 µg/ml | $364.00 | |||
HRPAP20 Antibody (G-10) Alexa Fluor® 790 | sc-166928 AF790 | 200 µg/ml | $364.00 |