HPV16 E7 Antibody (ED17): sc-6981

HPV16 E7 Antibody (ED17) is a mouse monoclonal IgG1 kappa light chain antibody that detects the HPV16 E7 protein of HPV-16 origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HPV16 E7 antibody (ED17) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPV16 E7 is a small zinc-binding phosphoprotein that plays a critical role in the regulation of the cell cycle by interacting with the retinoblastoma (pRb) tumor suppressor protein. This interaction leads to the disruption of pRb′s function, promoting uncontrolled cell proliferation, which is a hallmark of cancer. HPV16 E7′s ability to bind pRb is facilitated by the presence of the LXCXE motif, a sequence that is also found in other viral oncoproteins, such as the E1A protein of adenoviruses. Notably, high-risk HPV E7 proteins, including HPV16 E7, exhibit a significantly higher affinity for pRb compared to their low-risk counterparts, which underscores their role in oncogenesis. HPV16 E7 is predominantly localized in the nucleus, where HPV16 E7 exerts effects on cell cycle regulation, and HPV16 E7′s association with pRb not only promotes cell proliferation but also leads to the degradation of Rb family proteins, further enhancing oncogenic potential. Given the strong association of HPV16 and HPV18 with cervical, vaginal, and vulvar malignancies, HPV16 E7 monoclonal antibody (ED17) serves as a valuable tool for research into HPV-related cancers and the underlying mechanisms of viral oncogenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HPV16 E7 Antibody (ED17) References:

1. Association between poor prognosis in early-stage invasive cervical carcinomas and non-detection of HPV DNA.  |  Riou, G., et al. 1990. Lancet. 335: 1171-4. PMID: 1971033
2. Association of human papillomavirus types 16 and 18 E6 proteins with p53.  |  Werness, BA., et al. 1990. Science. 248: 76-9. PMID: 2157286
3. The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes.  |  Münger, K., et al. 1989. J Virol. 63: 4417-21. PMID: 2476573
4. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes.  |  Hawley-Nelson, P., et al. 1989. EMBO J. 8: 3905-10. PMID: 2555178
5. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product.  |  Münger, K., et al. 1989. EMBO J. 8: 4099-105. PMID: 2556261
6. The association of mammalian DREAM complex and HPV16 E7 proteins.  |  Rashid, NN., et al. 2015. Am J Cancer Res. 5: 3525-33. PMID: 26885443
7. Serum HPV16 E7 Oncoprotein Is a Recurrence Marker of Oropharyngeal Squamous Cell Carcinomas.  |  Oton-Gonzalez, L., et al. 2021. Cancers (Basel). 13: PMID: 34282779
8. MPS1 is involved in the HPV16-E7-mediated centrosomes amplification.  |  Alfaro-Mora, Y., et al. 2021. Cell Div. 16: 6. PMID: 34736484
9. Photosensitizer-induced HPV16 E7 nanovaccines for cervical cancer immunotherapy.  |  Zhang, L., et al. 2022. Biomaterials. 282: 121411. PMID: 35189461
10. The expression of Oct3/4A mRNA and not its isoforms is upregulated by the HPV16 E7 oncoprotein.  |  Gómez-Gómez, Y., et al. 2023. Mol Biol Rep. 50: 981-991. PMID: 36378419
11. Two distinct mechanisms regulate the levels of a cellular tumor antigen, p53.  |  Reich, NC., et al. 1983. Mol Cell Biol. 3: 2143-50. PMID: 6318085
12. Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53.  |  Huibregtse, JM., et al. 1993. Mol Cell Biol. 13: 775-84. PMID: 8380895

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPV16 E7 Antibody (ED17) has 173 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPV16 E7 Antibody (ED17) Specifications

Alternate Product Name: HPV16 E7

Product Clone Name: ED17

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-98

Epitope Target: E7

Epitope Species: HPV16

Product Reactivity/Detection: E7

Species Reactivity/Detection: HPV-16

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 173 citations in various scientific publications

Available Conjugates/Forms for HPV16 E7 Antibody (ED17): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HPV16 E7 antibody is HPV16 E7 Antibody (NM2): sc-65711.