HPV16 E7 Antibody (NM2): sc-65711
- HPV16 E7 Antibody (NM2) is a mouse monoclonal IgG2a κ, cited in 40 publications, provided at 200 µg/ml
- raised against amino acids 35-56 of E7 of HPV16 origin
- HPV16 E7 Antibody (NM2) is recommended for detection of E7 GST of HPV-16 origin by WB, IP and IF
- Anti-HPV16 E7 Antibody (NM2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for HPV16 E7 Antibody (NM2) for WB applications. These reagents are now offered in bundles with HPV16 E7 Antibody (NM2) (see ordering information below).
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SEE ALSO...
HPV16 E7 Antibody (NM2) is a mouse monoclonal IgG2a kappa light chain antibody that detects the E7 protein of HPV-16 origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). HPV16 E7 (NM2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The E7 protein is a small zinc-binding phosphoprotein that plays a crucial role in the regulation of the cell cycle by interacting with the retinoblastoma tumor suppressor protein (pRb). This interaction leads to the disruption of pRb′s function, promoting uncontrolled cell proliferation, which is a hallmark of cancer. The E7 protein′s ability to bind pRb is mediated by the LXCXE motif located within the CR2 homology region, which is also found in other viral oncoproteins, such as the E1A protein of adenoviruses. High-risk HPV E7 proteins, including HPV16 E7, exhibit a ten-fold greater affinity for pRb compared to their low-risk counterparts, thereby enhancing their oncogenic potential. The CR2 region contains a phosphorylation site for casein kinase II, which may further influence E7 protein′s activity and interactions. Given the strong association of HPV16 and HPV18 with cervical, vaginal, and vulvar malignancies, anti-HPV16 E7 antibody (NM2) serves as a valuable tool for research into HPV-related cancers and the underlying mechanisms of tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HPV16 E7 Antibody (NM2) | sc-65711 | 200 µg/ml | $322.00 | |||
HPV16 E7 Antibody (NM2): m-IgG Fc BP-HRP Bundle | sc-525683 | 200 µg Ab; 10 µg BP | $361.00 | |||
HPV16 E7 Antibody (NM2): m-IgG2a BP-HRP Bundle | sc-546363 | 200 µg Ab; 10 µg BP | $361.00 | |||
HPV16 E7 Antibody (NM2) AC | sc-65711 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HPV16 E7 Antibody (NM2) HRP | sc-65711 HRP | 200 µg/ml | $322.00 | |||
HPV16 E7 Antibody (NM2) FITC | sc-65711 FITC | 200 µg/ml | $336.00 | |||
HPV16 E7 Antibody (NM2) PE | sc-65711 PE | 200 µg/ml | $349.00 | |||
HPV16 E7 Antibody (NM2) Alexa Fluor® 488 | sc-65711 AF488 | 200 µg/ml | $364.00 | |||
HPV16 E7 Antibody (NM2) Alexa Fluor® 546 | sc-65711 AF546 | 200 µg/ml | $364.00 | |||
HPV16 E7 Antibody (NM2) Alexa Fluor® 594 | sc-65711 AF594 | 200 µg/ml | $364.00 | |||
HPV16 E7 Antibody (NM2) Alexa Fluor® 647 | sc-65711 AF647 | 200 µg/ml | $364.00 | |||
HPV16 E7 Antibody (NM2) Alexa Fluor® 680 | sc-65711 AF680 | 200 µg/ml | $364.00 | |||
HPV16 E7 Antibody (NM2) Alexa Fluor® 790 | sc-65711 AF790 | 200 µg/ml | $364.00 |