HPK1 Antibody (G-9): sc-374183
- HPK1 Antibody (G-9) is a mouse monoclonal IgG1 κ HPK1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against a peptide mapping at the C-terminus of HPK1 of human origin
- HPK1 Antibody (G-9) is recommended for detection of HPK1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-HPK1 Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HPK1 Antibody (G-9) for WB applications. These reagents are now offered in bundles with HPK1 Antibody (G-9) (see ordering information below).
QUICK LINKS
HPK1 Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects HPK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HPK1 (G-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. HPK1, or hematopoietic progenitor kinase 1, is crucial for regulating the c-Jun N-terminal kinase (JNK) signaling pathway, which is involved in cellular responses to stress and apoptosis. Located primarily in the cytoplasm, hematopoietic progenitor kinase 1 plays a significant role in mediating signals from various receptors, thereby influencing cell survival and differentiation. HPK1′s ability to phosphorylate MEKK highlights its importance in the stress-responsive JNK/SAPK signaling cascade, which is vital for cellular adaptation to environmental changes. Understanding hematopoietic progenitor kinase 1′s function and regulation is essential for elucidating its role in various physiological and pathological processes, including immune responses and cancer progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HPK1 Antibody (G-9) References:
- An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. | Wood, MA., et al. 2000. Mol Cell. 5: 321-30. PMID: 10882073
- Hematopoietic Progenitor Kinase1 (HPK1) Mediates T Cell Dysfunction and Is a Druggable Target for T Cell-Based Immunotherapies. | Si, J., et al. 2020. Cancer Cell. 38: 551-566.e11. PMID: 32860752
- Novel Substituted Exomethylene-oxindoles as HPK1 Inhibitors. | Sabnis, RW. 2021. ACS Med Chem Lett. 12: 681-682. PMID: 34055207
- The development of small-molecule inhibitors targeting HPK1. | Zhou, L., et al. 2022. Eur J Med Chem. 244: 114819. PMID: 36209628
- Current strategies for targeting HPK1 in cancer and the barriers to preclinical progress. | Chen, H., et al. 2024. Expert Opin Ther Targets. 28: 237-250. PMID: 38650383
- HPK1 citron homology domain regulates phosphorylation of SLP76 and modulates kinase domain interaction dynamics. | Chitre, AS., et al. 2024. Nat Commun. 15: 3725. PMID: 38697971
- HPK1 Dysregulation-Associated NK Cell Dysfunction and Defective Expansion Promotes Metastatic Melanoma Progression. | Choi, WS., et al. 2024. Adv Sci (Weinh). 11: e2400920. PMID: 38828677
- Opportunities and challenges for targeting HPK1 in cancer immunotherapy. | Xu, J., et al. 2024. Bioorg Chem. 153: 107866. PMID: 39369461
- Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. | Wu, C., et al. 1995. J Biol Chem. 270: 15984-92. PMID: 7608157
- Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. | Hu, MC., et al. 1996. Genes Dev. 10: 2251-64. PMID: 8824585
- NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain. | Su, YC., et al. 1997. EMBO J. 16: 1279-90. PMID: 9135144
- SH2/SH3 adaptor proteins can link tyrosine kinases to a Ste20-related protein kinase, HPK1. | Anafi, M., et al. 1997. J Biol Chem. 272: 27804-11. PMID: 9346925
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HPK1 Antibody (G-9) | sc-374183 | 200 µg/ml | $322.00 | |||
HPK1 Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-529685 | 200 µg Ab; 10 µg BP | $361.00 | |||
HPK1 Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-522654 | 200 µg Ab, 40 µg BP | $361.00 | |||
HPK1 Antibody (G-9) AC | sc-374183 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HPK1 Antibody (G-9) HRP | sc-374183 HRP | 200 µg/ml | $322.00 | |||
HPK1 Antibody (G-9) FITC | sc-374183 FITC | 200 µg/ml | $336.00 | |||
HPK1 Antibody (G-9) PE | sc-374183 PE | 200 µg/ml | $349.00 | |||
HPK1 Antibody (G-9) Alexa Fluor® 488 | sc-374183 AF488 | 200 µg/ml | $364.00 | |||
HPK1 Antibody (G-9) Alexa Fluor® 546 | sc-374183 AF546 | 200 µg/ml | $364.00 | |||
HPK1 Antibody (G-9) Alexa Fluor® 594 | sc-374183 AF594 | 200 µg/ml | $364.00 | |||
HPK1 Antibody (G-9) Alexa Fluor® 647 | sc-374183 AF647 | 200 µg/ml | $364.00 | |||
HPK1 Antibody (G-9) Alexa Fluor® 680 | sc-374183 AF680 | 200 µg/ml | $364.00 | |||
HPK1 Antibody (G-9) Alexa Fluor® 790 | sc-374183 AF790 | 200 µg/ml | $364.00 | |||
HPK1 (G-9) Neutralizing Peptide | sc-374183 P | 100 µg/0.5 ml | $69.00 |