HPA1 Antibody (E-10): sc-515935

HPA1 Antibody (E-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects HPA1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HPA1 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPA1, also known as heparanase-1, plays a crucial role in degrading heparan sulfate side chains of heparan sulfate proteoglycans in the extracellular matrix, which is vital for processes such as tumor metastasis and inflammation. By dismantling the subendothelial basal membrane, HPA1 facilitates the extravasation of blood-borne tumor cells and inflammatory leukocytes, thereby promoting angiogenesis and tumor vascularization. Additionally, proteolytic processing of HPA1 at the cell surface generates an active form that can be endocytosed by fibroblasts, leading to cytoplasmic accumulation. The gene encoding human HPA1 is located on chromosome 4q21.3, and HPA1′s ability to release growth factors and cytokines upon degradation underscores its importance in cell proliferation and chemotaxis, making HPA1 a significant target for research in cancer biology and therapeutic interventions.

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HPA1 Antibody (E-10) References:

1. Human HPA endoglycosidase heparanase. Map position 4q21.3.  |  Baker, E., et al. 1999. Chromosome Res. 7: 319. PMID: 10461877
2. Heparanase, a potential regulator of cell-matrix interactions.  |  Dempsey, LA., et al. 2000. Trends Biochem Sci. 25: 349-51. PMID: 10916150
3. Activation, processing and trafficking of extracellular heparanase by primary human fibroblasts.  |  Nadav, L., et al. 2002. J Cell Sci. 115: 2179-87. PMID: 11973358
4. Properties and function of heparanase in cancer metastasis and angiogenesis.  |  Vlodavsky, I. and Goldshmidt, O. 2001. Haemostasis. 31 Suppl 1: 60-3. PMID: 11990483
5. Expression of heparanase by platelets and circulating cells of the immune system: possible involvement in diapedesis and extravasation.  |  Vlodavsky, I., et al. 1992. Invasion Metastasis. 12: 112-27. PMID: 1399400
6. Establishment of an HPA-1- to -16-typed platelet donor registry in China.  |  Feng, ML., et al. 2006. Transfus Med. 16: 369-74. PMID: 16999761
7. Extracellular matrix-resident growth factors and enzymes: possible involvement in tumor metastasis and angiogenesis.  |  Vlodavsky, I., et al. 1990. Cancer Metastasis Rev. 9: 203-26. PMID: 1705486
8. Basic fibroblast growth factor binds to subendothelial extracellular matrix and is released by heparitinase and heparin-like molecules.  |  Bashkin, P., et al. 1989. Biochemistry. 28: 1737-43. PMID: 2541764
9. Noninvasive prenatal HPA-1 typing in HPA-1a negative pregnancies selected in the Polish PREVFNAIT screening program.  |  Orzińska, A., et al. 2018. Transfusion. 58: 2705-2711. PMID: 30260485
10. Validating the HPA-1 to -5 and -15 Detection by Homemade PCR-SSP, Real-Time PCR, and PCR-RFLP Methods.  |  Ghader Azizi, S., et al. 2022. Lab Med. 53: 570-579. PMID: 35689446
11. Lymphoma cell-mediated degradation of sulfated proteoglycans in the subendothelial extracellular matrix: relationship to tumor cell metastasis.  |  Vlodavsky, I., et al. 1983. Cancer Res. 43: 2704-11. PMID: 6601984
12. HPA-1 genotype in arterial thrombosis--role of HPA-1b polymorphism in platelet function.  |  Corral, J., et al. 1997. Blood Coagul Fibrinolysis. 8: 284-90. PMID: 9282792

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPA1 Antibody (E-10) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HPA1 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPA1 Antibody (E-10) Specifications

Alternate Product Name: Heparanase

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 491-535

Epitope Target: HPA1

Epitope Species: mouse

Product Reactivity/Detection: HPA1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HPA1 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HPA1 antibody is HPA1 Antibody (D-3): sc-518151.