HPA1 Antibody (D-3): sc-518151

HPA1 Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects HPA1 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HPA1 (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPA1 plays a crucial role in the degradation of heparan sulfate side chains of heparan sulfate proteoglycans in the extracellular matrix, which is vital for processes such as tumor metastasis and inflammation. By dismantling the subendothelial basal membrane, HPA1 facilitates the extravasation of blood-borne tumor cells and inflammatory leukocytes, thereby promoting angiogenesis and enhancing tumor vascularization. Additionally, the degradation of heparan sulfate proteoglycans by HPA1 releases growth factors and cytokines that stimulate cell proliferation and chemotaxis, further contributing to tumor progression. Notably, fibroblasts can endocytose extracellular HPA1, leading to its accumulation in the cytoplasm, which underscores its significance in cellular signaling and interaction. The gene encoding human HPA1 is located on chromosome 4q21.3, highlighting its genetic importance in various biological processes.

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HPA1 Antibody (D-3) References:

1. A rapid and specific whole blood HPA-1 phenotyping by flow cytometry using two commercialized monoclonal antibodies directed against GP IIIa and GP IIb-IIIa complexes.  |  Sorel, N., et al. 2004. Br J Haematol. 124: 221-3. PMID: 14687033
2. Platelet GP IIIa polymorphism HPA-1 (PlA) protects against subarachnoid hemorrhage.  |  Iniesta, JA., et al. 2004. Stroke. 35: 2282-6. PMID: 15331787
3. Real-time PCR genotyping of human platelet alloantigens HPA-1, HPA-2, HPA-3 and HPA-5 is superior to the standard PCR-SSP method.  |  Ficko, T., et al. 2004. Transfus Med. 14: 425-32. PMID: 15569237
4. Reactivity of T cells from women with antibodies to the human platelet antigen (HPA)-1a to peptides encompassing the HPA-1 polymorphism.  |  Jackson, DJ., et al. 2005. Clin Exp Immunol. 142: 92-102. PMID: 16178861
5. DNA sequencing-based typing of HPA-1 to HPA-17w systems.  |  Wu, GG., et al. 2008. Int J Hematol. 88: 268-271. PMID: 18802675
6. Platelet HPA-1 a/HPA-1 b polymorphism and the risk of periprocedural myocardial infarction in patients undergoing elective PCI.  |  Verdoia, M., et al. 2014. Platelets. 25: 367-72. PMID: 24283589
7. Increased levels of β-catenin, LEF-1, and HPA-1 correlate with poor prognosis for acral melanoma with negative BRAF and NRAS mutation in BRAF exons 11 and 15 and NRAS exons 1 and 2.  |  Xu, S., et al. 2015. DNA Cell Biol. 34: 69-77. PMID: 25343173
8. Frequency of human platelet antigens (HPA)-1, -2, -5 and -15 in Brazilian blood donors and establishment of a panel of HPA-typed donors.  |  Mangerona, CM., et al. 2015. Transfus Med. 25: 189-94. PMID: 26033262
9. HPA-1 typing by PCR amplification with sequence-specific primers (PCR-SSP): a rapid and simple technique.  |  Metcalfe, P. and Waters, AH. 1993. Br J Haematol. 85: 227-9. PMID: 8251400
10. Flow cytometry immunophenotyping and polymerase chain reaction-site-specific primers genotyping for HPA-1 alloantigens in an Italian blood donor population.  |  Tazzari, PL., et al. 1998. Vox Sang. 74: 42-5. PMID: 9481860

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPA1 Antibody (D-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-HPA1 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPA1 Antibody (D-3) Specifications

Alternate Product Name: HPA1

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 82-104

Epitope Target: HPA1

Epitope Species: mouse

Product Reactivity/Detection: HPA1

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for HPA1 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin