HPA1 Antibody (B-4): sc-518153

HPA1 Antibody (B-4) is a mouse monoclonal IgG2a antibody that detects heparanase 1 (HPA1) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HPA1 plays a crucial role in the degradation of heparan sulfate side chains of heparan sulfate proteoglycans in the extracellular matrix, which is vital for various physiological and pathological processes, including tumor metastasis and inflammation. By dismantling the subendothelial basal membrane, HPA1 facilitates the extravasation of blood-borne tumor cells and inflammatory leukocytes, thereby promoting cancer progression and angiogenesis. HPA1′s ability to release growth factors and cytokines upon degradation further stimulates cell proliferation and chemotaxis, highlighting HPA1′s importance in tumor vascularization. Additionally, fibroblasts can endocytose extracellular HPA1, leading to accumulation in the cytoplasm, which may influence cellular responses to the tumor microenvironment. The gene encoding human HPA1 is located on chromosome 4q21.3, underscoring HPA1′s significance in human health and disease. Anti-HPA1 antibody (B-4) is an essential tool for researchers investigating the roles of heparanase in cancer biology and inflammation.

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HPA1 Antibody (B-4) References:

1. HPA-1, 3, 5 genotyping to establish a typed platelet donor panel.  |  Verran, J., et al. 2000. Pathology. 32: 89-93. PMID: 10840826
2. Comparison of human platelet antigen (HPA)-1a typing by solid phase red cell adherence to HPA-1 allotypes determined by allele-specific restriction enzyme analysis.  |  McGann, MJ., et al. 2000. Immunohematology. 16: 68-73. PMID: 15373621
3. HPA-1 polymorphism of alphaIIbbeta3 modulates platelet adhesion onto immobilized fibrinogen in an in-vitro flow system.  |  Loncar, R., et al. 2007. Thromb J. 5: 2. PMID: 17309793
4. Gene frequencies of the HPA-1 platelet antigen alleles in the Lebanese population.  |  Sabbagh, AS., et al. 2007. Transfus Med. 17: 473-8. PMID: 18067652
5. Allelic frequencies of HPA-1 to 5 human platelet antigens in patients infected with hepatitis C virus.  |  Verdichio-Moraes, CF., et al. 2009. J Med Virol. 81: 757-9. PMID: 19235843
6. Expression and clinical significance of syndecan-1 mRNA and HPA-1 mRNA in colorectal cancer detected with real-time fluorescent quantitative polymerase chain reaction.  |  Wang, H., et al. 2010. Chin J Cancer. 29: 288-93. PMID: 20193112
7. New mutation in the beta 3-integrin (GPIIIa) gene inducing HPA-1 genotyping discrepancies.  |  Bertrand, G., et al. 2010. Transfusion. 50: 1589-91. PMID: 20636532
8. Comparison of a simple-probe real-time PCR and multiplex PCR techniques for HPA-1 to HPA-6 and HPA-15 genotyping.  |  Kengkate, M., et al. 2015. J Clin Lab Anal. 29: 94-9. PMID: 24687514
9. Genotyping provides a reliable tool for the determination of the platelet antigen system HPA-1 in Glanzmann's thrombasthenia.  |  Pober, M., et al. 1993. Br J Haematol. 85: 112-5. PMID: 8251377
10. One-tube method for complete HPA-1 genotyping by PCR using sequence-specific primers.  |  Boldt, B., et al. 1997. Br J Haematol. 99: 968-73. PMID: 9432051

The preferred HPA1 antibody is HPA1 Antibody (D-3): sc-518151.