HP1β Antibody (4D7B8): sc-517288
- HP1β Antibody (4D7B8) is a mouse monoclonal IgG1 κ HP1β antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-185 of HP1β of human origin
- HP1 beta Antibody (4D7B8) is recommended for detection of HP1β of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
- Anti-HP1 beta Antibody (4D7B8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HP1β Antibody (4D7B8) for WB and IHC(P) applications. These reagents are now offered in bundles with HP1β Antibody (4D7B8) (see ordering information below).
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HP1β Antibody (4D7B8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HP1 beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-HP1β antibody (4D7B8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HP1 beta protein plays a crucial role in heterochromatin formation and maintenance, essential for regulating gene expression and maintaining genomic stability. Heterochromatin is characterized by densely packed structure, which limits access to transcription machinery, thereby silencing gene expression in these regions. HP1β interaction with chromatin assembly factor-1 (CAF-1) facilitates newly synthesized histone deposition into chromatin during DNA replication. This process ensures accurate genetic material replication and proper organization, critical for cell division and prevention of genomic instability. Dynamic association of HP1 proteins with chromatin during cell cycle, particularly reassociation at mitosis end, underscores their importance in maintaining chromatin architecture and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HP1β Antibody (4D7B8) References:
- Methylation-independent binding to histone H3 and cell cycle-dependent incorporation of HP1beta into heterochromatin. | Dialynas, GK., et al. 2006. J Biol Chem. 281: 14350-60. PMID: 16547356
- Regulation of an inducible promoter by an HP1beta-HP1gamma switch. | Mateescu, B., et al. 2008. EMBO Rep. 9: 267-72. PMID: 18239689
- A method for systematic mapping of protein lysine methylation identifies functions for HP1β in DNA damage response. | Liu, H., et al. 2013. Mol Cell. 50: 723-35. PMID: 23707759
- Characterization of the effects of phosphorylation by CK2 on the structure and binding properties of human HP1β. | Munari, F., et al. 2014. FEBS Lett. 588: 1094-9. PMID: 24561199
- Epigenome rejuvenation: HP1β mobility as a measure of pluripotent and senescent chromatin ground states. | Manukyan, M. and Singh, PB. 2014. Sci Rep. 4: 4789. PMID: 24763337
- HP1β suppresses metastasis of human cancer cells by decreasing the expression and activation of MMP2. | Yi, SA., et al. 2014. Int J Oncol. 45: 2541-8. PMID: 25201136
- HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs. | Stixová, L., et al. 2014. Epigenetics Chromatin. 7: 39. PMID: 25587355
- HP1β is a biomarker for breast cancer prognosis and PARP inhibitor therapy. | Lee, YH., et al. 2015. PLoS One. 10: e0121207. PMID: 25769025
- Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells. | Mattout, A., et al. 2015. Genome Biol. 16: 213. PMID: 26415775
- The level and distribution pattern of HP1β in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3. | Bártová, E., et al. 2016. Histochem Cell Biol. 145: 447-61. PMID: 26794325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HP1β Antibody (4D7B8) | sc-517288 | 200 µg/ml | $316.00 | |||
HP1β Antibody (4D7B8): m-IgG Fc BP-HRP Bundle | sc-528074 | 200 µg Ab; 10 µg BP | $354.00 | |||
HP1β Antibody (4D7B8): m-IgG1 BP-HRP Bundle | sc-533447 | 200 µg Ab; 20 µg BP | $354.00 | |||
HP1β Antibody (4D7B8) AC | sc-517288 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HP1β Antibody (4D7B8) HRP | sc-517288 HRP | 200 µg/ml | $316.00 | |||
HP1β Antibody (4D7B8) FITC | sc-517288 FITC | 200 µg/ml | $330.00 | |||
HP1β Antibody (4D7B8) PE | sc-517288 PE | 200 µg/ml | $343.00 | |||
HP1β Antibody (4D7B8) Alexa Fluor® 488 | sc-517288 AF488 | 200 µg/ml | $357.00 | |||
HP1β Antibody (4D7B8) Alexa Fluor® 546 | sc-517288 AF546 | 200 µg/ml | $357.00 | |||
HP1β Antibody (4D7B8) Alexa Fluor® 594 | sc-517288 AF594 | 200 µg/ml | $357.00 | |||
HP1β Antibody (4D7B8) Alexa Fluor® 647 | sc-517288 AF647 | 200 µg/ml | $357.00 | |||
HP1β Antibody (4D7B8) Alexa Fluor® 680 | sc-517288 AF680 | 200 µg/ml | $357.00 | |||
HP1β Antibody (4D7B8) Alexa Fluor® 790 | sc-517288 AF790 | 200 µg/ml | $357.00 |