HP1 Antibody (E-6): sc-515341

HP1 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects HP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HP1 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HP1, also known as heterochromatin protein 1, plays a crucial role in the formation and maintenance of heterochromatin, which is essential for gene regulation and chromatin structure. Heterochromatin is characterized by its densely packed structure, which limits access to transcription machinery, thereby silencing gene expression in these regions. This silencing is vital for maintaining genomic stability and regulating developmental processes. HP1 interacts with various chromatin-associated factors, including chromatin assembly factor-1 (CAF-1), which is essential for the deposition of newly synthesized histones during DNA replication. The dynamic association of HP1 with chromatin during the cell cycle, particularly reassociation at the end of mitosis, underscores its importance in epigenetic regulation of gene expression and preservation of chromatin integrity.

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HP1 Antibody (E-6) References:

1. Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.  |  Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
2. Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins.  |  Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
3. Duplication and maintenance of heterochromatin domains.  |  Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
4. Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1).  |  Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
5. Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro.  |  Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
6. How HP1 Post-Translational Modifications Regulate Heterochromatin Formation and Maintenance.  |  Sales-Gil, R. and Vagnarelli, P. 2020. Cells. 9: PMID: 32545538
7. Functions of HP1 proteins in transcriptional regulation.  |  Schoelz, JM. and Riddle, NC. 2022. Epigenetics Chromatin. 15: 14. PMID: 35526078
8. HP1-Driven Micro-Phase Separation of Heterochromatin-Like Domains/Complexes.  |  Singh, PB. and Newman, AG. 2022. Epigenet Insights. 15: 25168657221109766. PMID: 35813402
9. A complex interplay between H2A.Z and HP1 isoforms regulates pericentric heterochromatin.  |  González, J., et al. 2023. Front Cell Dev Biol. 11: 1293122. PMID: 38020886
10. Evolutionary adaptation of an HP1-protein chromodomain integrates chromatin and DNA sequence signals.  |  Baumgartner, L., et al. 2024. Elife. 13: PMID: 38995818
11. The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication.  |  Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
12. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HP1 Antibody (E-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-HP1 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HP1 Antibody (E-6) Specifications

Alternate Product Name: pan Chromobox

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-191

Epitope Target: HP1α

Epitope Species: human

Product Reactivity/Detection: HP1α, HP1β and HP1γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for HP1 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin