HP1α Antibody (GA-62): sc-130446

HP1α Antibody (GA-62) is a mouse monoclonal IgG1 kappa light chain antibody that detects HP1 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HP1α antibody (GA-62) is available as a non-conjugated format. HP1 alpha protein plays a crucial role in heterochromatin formation and maintenance, essential for regulating gene expression and maintaining genomic stability. Heterochromatin is characterized by its densely packed structure, which limits access to transcription machinery, thereby silencing gene expression in these regions. This silencing is vital for processes such as X-chromosome inactivation and transposable element suppression, which can otherwise lead to genomic instability. HP1α interaction with chromatin assembly factor-1 (CAF-1) facilitates newly synthesized histone deposition into chromatin during DNA replication, ensuring faithful inheritance of genome epigenetic state. Dynamic association of HP1 proteins with chromatin during cell cycle highlights their role in chromatin remodeling and gene expression regulation, making HP1α a significant marker for chromatin biology and epigenetics studies.

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HP1α Antibody (GA-62) References:

1. Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.  |  Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
2. Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins.  |  Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
3. Duplication and maintenance of heterochromatin domains.  |  Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
4. Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1).  |  Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
5. Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro.  |  Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
6. Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin.  |  Larson, AG., et al. 2017. Nature. 547: 236-240. PMID: 28636604
7. Changes in the properties of membrane tethers in response to HP1α depletion in MCF7 cells.  |  Pradhan, S., et al. 2022. Biochem Biophys Res Commun. 587: 126-130. PMID: 34872000
8. Phosphorylated HP1α-Nucleosome Interactions in Phase Separated Environments.  |  Elathram, N., et al. 2023. J Am Chem Soc. 145: 23994-24004. PMID: 37870432
9. Unveiling the role of HP1α-HDAC1-STAT1 axis as a therapeutic target for HP1α-positive intrahepatic cholangiocarcinoma.  |  Xiong, F., et al. 2024. J Exp Clin Cancer Res. 43: 152. PMID: 38812060
10. The condensation of HP1α/Swi6 imparts nuclear stiffness.  |  Williams, JF., et al. 2024. Cell Rep. 43: 114373. PMID: 38900638
11. The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication.  |  Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
12. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HP1α Antibody (GA-62) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-HP1α Antibody (GA-62) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HP1α Antibody (GA-62) Specifications

Alternate Product Name: CBX5

Product Clone Name: GA-62

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-192

Epitope Target: HP1α

Epitope Species: human

Product Reactivity/Detection: HP1α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for HP1α Antibody (GA-62): N/A

The preferred HP1 antibody is HP1 Antibody (E-6): sc-515341.