HoxB9 Antibody (H-8): sc-398500

HoxB9 Antibody (H-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects HoxB9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HoxB9 antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HoxB9 plays a crucial role in embryonic development and cellular differentiation by regulating downstream target genes, which is essential for proper morphogenesis along the anterior-posterior body axis. Specifically, HoxB9 directs DNA-protein and protein-protein interactions vital for establishing structural characteristics of developing tissues. The mammalian HOX gene complex comprises 39 genes distributed across four linkage groups on four chromosomes, with the HoxB cluster in mice containing HoxB1 to HoxB9 and HoxB13, all transcribed in the same direction. HoxB9 interacts with transcriptional cofactors BTG1 and BTG2, which enhance transcriptional activity. Dysregulation of HoxB9 expression has been linked to various forms of leukemia, highlighting its importance in both normal development and disease.

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HoxB9 Antibody (H-8) References:

1. The leukemia-associated protein Btg1 and the p53-regulated protein Btg2 interact with the homeoprotein Hoxb9 and enhance its transcriptional activation.  |  Prévôt, D., et al. 2000. J Biol Chem. 275: 147-53. PMID: 10617598
2. A large targeted deletion of Hoxb1-Hoxb9 produces a series of single-segment anterior homeotic transformations.  |  Medina-Martínez, O., et al. 2000. Dev Biol. 222: 71-83. PMID: 10885747
3. Altered HOX and WNT7A expression in human lung cancer.  |  Calvo, R., et al. 2000. Proc Natl Acad Sci U S A. 97: 12776-81. PMID: 11070089
4. Expression of 11 HOX genes is deregulated in esophageal squamous cell carcinoma.  |  Chen, KN., et al. 2005. Clin Cancer Res. 11: 1044-9. PMID: 15709170
5. Elevated HOXB9 expression promotes differentiation and predicts a favourable outcome in colon adenocarcinoma patients.  |  Zhan, J., et al. 2014. Br J Cancer. 111: 883-93. PMID: 25025961
6. An E2F1-HOXB9 transcriptional circuit is associated with breast cancer progression.  |  Zhussupova, A., et al. 2014. PLoS One. 9: e105285. PMID: 25136922
7. HoxB9 promotes the migration and invasion via TGF-β1/Smad2/Slug signaling pathway in oral squamous cell carcinoma.  |  Xue, M., et al. 2017. Am J Transl Res. 9: 1151-1161. PMID: 28386341
8. Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal adenocarcinoma.  |  Sun, X., et al. 2020. Front Med. 14: 91-100. PMID: 31372881
9. Identification of HOXB9 to predict prognosis of endometrial cancer based on comprehensive bioinformatics analysis.  |  Xu, Y., et al. 2023. Eur J Med Res. 28: 79. PMID: 36803556
10. HOXB9 promotes laryngeal squamous cell carcinoma progression by upregulating MMP12.  |  Sun, C., et al. 2024. Funct Integr Genomics. 24: 78. PMID: 38632141
11. Paralogous mouse Hox genes, Hoxa9, Hoxb9, and Hoxd9, function together to control development of the mammary gland in response to pregnancy.  |  Chen, F. and Capecchi, MR. 1999. Proc Natl Acad Sci U S A. 96: 541-6. PMID: 9892669
12. Modulation of homeobox B6 and B9 genes expression in human leukemia cell lines during myelomonocytic differentiation.  |  Ohnishi, K., et al. 1998. Leuk Lymphoma. 31: 599-608. PMID: 9922051

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HoxB9 Antibody (H-8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-HoxB9 Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HoxB9 Antibody (H-8) Specifications

Alternate Product Name: HoxB9

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 101-180

Epitope Target: HoxB9

Epitope Species: human

Product Reactivity/Detection: HoxB9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for HoxB9 Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent