HOOK2 Antibody (G-4): sc-365716
- HOOK2 Antibody (G-4) is a mouse monoclonal IgG1 κ HOOK2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 151-185 within an internal region of HOOK2 of human origin
- HOOK2 Antibody (G-4) is recommended for detection of HOOK2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-HOOK2 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HOOK2 Antibody (G-4) for WB applications. These reagents are now offered in bundles with HOOK2 Antibody (G-4) (see ordering information below).
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HOOK2 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects HOOK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HOOK2 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HOOK2 protein, also known as HK2, is a 719 amino acid long homodimer that plays a crucial role in the spatial organization of membrane-trafficking systems by interacting with microtubules through its conserved N-terminal domain. This interaction is vital for maintaining cellular organization and facilitating organelle transport, which is essential for proper cellular function and communication. Unlike HOOK3, which is localized to the Golgi apparatus, HOOK2 is found in distinct subcellular structures that do not correspond to typical organelles such as early or late endosomes, mitochondria, or lysosomes, indicating a unique role in cellular dynamics. HOOK2 may also interact with proteins such as SURF1 and Zic2, suggesting potential involvement in esophageal cancer as a tumor antigen. HOOK2 expression is notably strong in the larynx and esophagus, highlighting its significance in these tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HOOK2 Antibody (G-4) References:
- The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. | Walenta, JH., et al. 2001. J Cell Biol. 152: 923-34. PMID: 11238449
- Serological identification of tumor antigens of esophageal squamous cell carcinoma. | Shimada, H., et al. 2005. Int J Oncol. 26: 77-86. PMID: 15586227
- A novel hook-related protein family and the characterization of hook-related protein 1. | Simpson, F., et al. 2005. Traffic. 6: 442-58. PMID: 15882442
- Hook2 contributes to aggresome formation. | Szebenyi, G., et al. 2007. BMC Cell Biol. 8: 19. PMID: 17540036
- An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex. | Xu, L., et al. 2008. Mol Biol Cell. 19: 5059-71. PMID: 18799622
- Genetic analysis of hook, a gene required for endocytic trafficking in drosophila. | Krämer, H. and Phistry, M. 1999. Genetics. 151: 675-84. PMID: 9927460
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HOOK2 Antibody (G-4) | sc-365716 | 200 µg/ml | $322.00 | |||
HOOK2 Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-529446 | 200 µg Ab; 10 µg BP | $361.00 | |||
HOOK2 Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-522339 | 200 µg Ab, 40 µg BP | $361.00 | |||
HOOK2 Antibody (G-4) AC | sc-365716 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HOOK2 Antibody (G-4) HRP | sc-365716 HRP | 200 µg/ml | $322.00 | |||
HOOK2 Antibody (G-4) FITC | sc-365716 FITC | 200 µg/ml | $336.00 | |||
HOOK2 Antibody (G-4) PE | sc-365716 PE | 200 µg/ml | $349.00 | |||
HOOK2 Antibody (G-4) Alexa Fluor® 488 | sc-365716 AF488 | 200 µg/ml | $364.00 | |||
HOOK2 Antibody (G-4) Alexa Fluor® 546 | sc-365716 AF546 | 200 µg/ml | $364.00 | |||
HOOK2 Antibody (G-4) Alexa Fluor® 594 | sc-365716 AF594 | 200 µg/ml | $364.00 | |||
HOOK2 Antibody (G-4) Alexa Fluor® 647 | sc-365716 AF647 | 200 µg/ml | $364.00 | |||
HOOK2 Antibody (G-4) Alexa Fluor® 680 | sc-365716 AF680 | 200 µg/ml | $364.00 | |||
HOOK2 Antibody (G-4) Alexa Fluor® 790 | sc-365716 AF790 | 200 µg/ml | $364.00 | |||
HOOK2 (G-4) Neutralizing Peptide | sc-365716 P | 100 µg/0.5 ml | $69.00 |