HOOK1 Antibody (E-6): sc-398233
- HOOK1 Antibody (E-6) is a mouse monoclonal IgG1 κ HOOK1 antibody provided at 200 µg/ml
- raised against amino acids 309-354 mapping within an internal region of HOOK1 of human origin
- HOOK1 Antibody (E-6) is recommended for detection of HOOK1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- Anti-HOOK1 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HOOK1 Antibody (E-6) for WB applications. These reagents are now offered in bundles with HOOK1 Antibody (E-6) (see ordering information below).
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HOOK1 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects HOOK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HOOK1 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HOOK1 protein plays a crucial role in organizing microtubules, which are essential for the spatial arrangement of membrane-trafficking systems within cells. As a cytoskeletal linker protein, HOOK1 is involved in endocytic membrane trafficking and exists as a homodimer, likely formed through central coiled-coil domain. HOOK1 protein interacts with VPS18, a component of the endosomal sorting complex required for transport, and is vital for spermatid differentiation, aiding in positioning manchette microtubules and flagellum. HOOK1 protein is primarily localized in cytoplasm and does not associate with Golgi complex, distinguishing HOOK1 from family member HOOK3, which is involved in organizing cis-Golgi compartment. These unique structural features and interactions of HOOK1 underscore HOOK1′s importance in cellular processes, making anti-HOOK1 antibody (E-6) an invaluable tool for researchers studying microtubule dynamics and membrane trafficking.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HOOK1 Antibody (E-6) References:
- Interconnections of CLN3, Hook1 and Rab proteins link Batten disease to defects in the endocytic pathway. | Luiro, K., et al. 2004. Hum Mol Genet. 13: 3017-27. PMID: 15471887
- Elevation of Hook1 in a disease model of Batten disease does not affect a novel interaction between Ankyrin G and Hook1. | Weimer, JM., et al. 2005. Biochem Biophys Res Commun. 330: 1176-81. PMID: 15823567
- A novel hook-related protein family and the characterization of hook-related protein 1. | Simpson, F., et al. 2005. Traffic. 6: 442-58. PMID: 15882442
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HOOK1 Antibody (E-6) | sc-398233 | 200 µg/ml | $316.00 | |||
HOOK1 Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-530723 | 200 µg Ab; 10 µg BP | $354.00 | |||
HOOK1 Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-524256 | 200 µg Ab, 40 µg BP | $354.00 | |||
HOOK1 Antibody (E-6): m-IgG1 BP-HRP Bundle | sc-544218 | 200 µg Ab; 20 µg BP | $354.00 | |||
HOOK1 Antibody (E-6) AC | sc-398233 AC | 500 µg/ml, 25% agarose | $416.00 | |||
HOOK1 Antibody (E-6) HRP | sc-398233 HRP | 200 µg/ml | $316.00 | |||
HOOK1 Antibody (E-6) FITC | sc-398233 FITC | 200 µg/ml | $330.00 | |||
HOOK1 Antibody (E-6) PE | sc-398233 PE | 200 µg/ml | $343.00 | |||
HOOK1 Antibody (E-6) Alexa Fluor® 488 | sc-398233 AF488 | 200 µg/ml | $357.00 | |||
HOOK1 Antibody (E-6) Alexa Fluor® 546 | sc-398233 AF546 | 200 µg/ml | $357.00 | |||
HOOK1 Antibody (E-6) Alexa Fluor® 594 | sc-398233 AF594 | 200 µg/ml | $357.00 | |||
HOOK1 Antibody (E-6) Alexa Fluor® 647 | sc-398233 AF647 | 200 µg/ml | $357.00 | |||
HOOK1 Antibody (E-6) Alexa Fluor® 680 | sc-398233 AF680 | 200 µg/ml | $357.00 | |||
HOOK1 Antibody (E-6) Alexa Fluor® 790 | sc-398233 AF790 | 200 µg/ml | $357.00 |