hnRNP M1-4 Antibody (1D8): sc-20002

hnRNP M1-4 Antibody (1D8) is a mouse monoclonal IgG1 kappa light chain antibody that detects hnRNP M1-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). hnRNP M1-4 (1D8) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Heterogeneous nuclear ribonucleoproteins (hnRNPs) play a crucial role in the regulation of gene expression, particularly in mRNA processing and transport. hnRNP M proteins, specifically, are involved in pre-mRNA splicing and can influence the stability and translation of mRNA. hnRNP M1-4 proteins are known to interact with other splicing factors and RNA-binding proteins, which is essential for the proper assembly of the spliceosome, a complex responsible for the removal of introns from pre-mRNA. This interaction is vital for maintaining the fidelity of gene expression, as errors in splicing can lead to the production of dysfunctional proteins and contribute to various diseases. The ability of hnRNP M proteins to shuttle between the nucleus and cytoplasm further underscores their importance in post-transcriptional regulation, making anti-hnRNP M1-4 monoclonal antibody (1D8) an invaluable tool for researchers studying RNA biology and its implications in health and disease.

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hnRNP M1-4 Antibody (1D8) References:

1. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
2. Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm.  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
3. The 72/74-kDa polypeptides of the 70-110 S large heterogeneous nuclear ribonucleoprotein complex (LH-nRNP) represent a discrete subset of the hnRNP M protein family.  |  Kafasla, P., et al. 2000. Biochem J. 350 Pt 2: 495-503. PMID: 10947964
4. Spatiotemporal regulation of hnRNP M and 2H9 gene expression during mouse embryonic development.  |  Mahé, D., et al. 2000. Biochim Biophys Acta. 1492: 414-24. PMID: 11004512
5. The alternative splicing repressors hnRNP A1/A2 and PTB influence pyruvate kinase isoform expression and cell metabolism.  |  Clower, CV., et al. 2010. Proc Natl Acad Sci U S A. 107: 1894-9. PMID: 20133837
6. Selective interactions of hnRNP M isoforms with the TET proteins TAF15 and TLS/FUS.  |  Marko, M., et al. 2014. Mol Biol Rep. 41: 2687-95. PMID: 24474660
7. LONRF2 is a protein quality control ubiquitin ligase whose deficiency causes late-onset neurological deficits.  |  Li, D., et al. 2023. Nat Aging. 3: 1001-1019. PMID: 37474791
8. A nuclear localization domain in the hnRNP A1 protein.  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
9. The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.  |  Datar, KV., et al. 1993. Nucleic Acids Res. 21: 439-46. PMID: 8441656
10. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
11. The human hnRNP-M proteins: structure and relation with early heat shock-induced splicing arrest and chromosome mapping.  |  Gattoni, R., et al. 1996. Nucleic Acids Res. 24: 2535-42. PMID: 8692693
12. mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end.  |  Ostareck, DH., et al. 1997. Cell. 89: 597-606. PMID: 9160751

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-hnRNP M1-4 Antibody (1D8) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-hnRNP M1-4 Antibody (1D8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

hnRNP M1-4 Antibody (1D8) Specifications

Alternate Product Name: hnRNP M1-4

Product Clone Name: 1D8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: hnRNP M1-4

Epitope Species: mouse

Product Reactivity/Detection: hnRNP M1, hnRNP M2, hnRNP M3 and hnRNP M4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: rabbit, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of KAPPA

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for hnRNP M1-4 Antibody (1D8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin