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hnRNP L Antibody (D-5): sc-48391

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Datasheets
  • hnRNP L Antibody (D-5) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
  • raised against amino acids 257-334 of hnRNP L of human origin
  • recommended for detection of hnRNP L of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
  • See hnRNP L (4D11): sc-32317 for hnRNP L antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for hnRNP L Antibody (D-5) for WB and IHC(P) applications. These reagents are now offered in bundles with hnRNP L Antibody (D-5) (see ordering information below).
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hnRNP L Antibody (D-5) is a mouse monoclonal IgG1 antibody that detects hnRNP L in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). hnRNP L plays a crucial role in the regulation of pre-mRNA splicing and is essential for proper mRNA processing, which is vital for gene expression and cellular function. hnRNP L is primarily localized in the nucleoplasm, where hnRNP L interacts with pre-mRNA to influence alternative splicing mechanisms, thereby affecting the diversity of protein isoforms produced from a single gene. hnRNP L′s ability to shuttle between the nucleus and cytoplasm also highlights hnRNP L′s importance in post-transcriptional regulation, as hnRNP L can impact mRNA stability and translation. Additionally, hnRNP L has been shown to interact with various other proteins, including hnRNP I, which further underscores hnRNP L′s role in the spliceosome complex and involvement in the translation of certain viruses, such as hepatitis C virus. anti-hnRNP L antibody (D-5) is an invaluable tool for researchers studying gene expression regulation, splicing mechanisms, and molecular pathways involved in viral infections.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

hnRNP L Antibody (D-5)

sc-48391
200 µg/ml
$322.00

hnRNP L Antibody (D-5): m-IgG Fc BP-HRP Bundle

sc-536825
200 µg Ab; 10 µg BP
$361.00

hnRNP L Antibody (D-5): m-IgGκ BP-HRP Bundle

sc-533992
200 µg Ab; 40 µg BP
$361.00

hnRNP L Antibody (D-5): m-IgG1 BP-HRP Bundle

sc-544843
200 µg Ab; 20 µg BP
$361.00

Can hnRNP L (D-5): sc-48391 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, hnRNP L (D-5): sc-48391 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
  • y_2026, m_4, d_9, h_7CST
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Rated 5 out of 5 by from The antibody works well for Western blottingThe antibody works well for Western blotting detecting endogenous hnRNP L from SHP-77 and HeLa whole cell lysates and Jurkat and NIH/3T3 nuclear extracts.
Date published: 2015-04-15
Rated 2 out of 5 by from Lots of background and multiple bands detectedLots of background and multiple bands detected with transfected lysate -SCBT QC
Date published: 2015-04-05
Rated 5 out of 5 by from Robust mmunoperoxidase staining of formalin fixedRobust mmunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells. - SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Great immunofluorescence staining of methanolGreat immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization. - SCBT QC
Date published: 2015-02-11
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hnRNP L Antibody (D-5) is rated 4.3 out of 5 by 4.
  • y_2026, m_4, d_9, h_7
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