hnRNP A/B Antibody (G-12): sc-390957
- hnRNP A/B Antibody (G-12) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1-36 mapping at the N-terminus of hnRNP A/B of mouse origin
- Anti-hnRNP A/B Antibody (G-12) is recommended for detection of hnRNP A/B of mouse and rat origin by WB, IP, IF and ELISA
- Anti-hnRNP A/B Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-390957 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for hnRNP A/B Antibody (G-12) for WB applications. These reagents are now offered in bundles with hnRNP A/B Antibody (G-12) (see ordering information below).
hnRNP A/B Antibody (G-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects hnRNP A/B in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-hnRNP A/B antibody (G-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Heterogeneous nuclear ribonucleoproteins (hnRNPs) play a crucial role in pre-mRNA processing and transport, binding to heterogeneous nuclear RNA (hnRNA) produced by RNA polymerase II. hnRNP A/B, also known as HNRNPAB, ABBP1, or HNRPAB, is a 332 amino acid nuclear protein widely expressed across various tissues. hnRNP A/B binds single-stranded RNA with high affinity for G-rich and U-rich regions of hnRNA, which is essential for gene expression regulation. hnRNP A/B contains two RNA recognition motif (RRM) domains, facilitating interaction with other proteins, including APOBEC1, an enzyme involved in mRNA editing. hnRNP A/B′s influence on pre-mRNA processing and mRNA metabolism underscores its significance in cellular function and gene regulation, making hnRNP A/B monoclonal antibody (G-12) an invaluable tool for researchers studying RNA biology and related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
hnRNP A/B Antibody (G-12) References:
- hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. | Caputi, M., et al. 1999. EMBO J. 18: 4060-7. PMID: 10406810
- Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. | Martinez-Contreras, R., et al. 2006. PLoS Biol. 4: e21. PMID: 16396608
- Multiple specificities of autoantibodies against hnRNP A/B proteins in systemic rheumatic diseases and hnRNP L as an associated novel autoantigen. | Siapka, S., et al. 2007. Autoimmunity. 40: 223-33. PMID: 17453722
- Functional implications of the emergence of alternative splicing in hnRNP A/B transcripts. | Han, SP., et al. 2010. RNA. 16: 1760-8. PMID: 20651029
- HNRNPAB-regulated lncRNA-ELF209 inhibits the malignancy of hepatocellular carcinoma. | Yang, Y., et al. 2020. Int J Cancer. 146: 169-180. PMID: 31090062
- Cellular hnRNPAB binding to viral nucleoprotein inhibits flu virus replication by blocking nuclear export of viral mRNA. | Wang, X., et al. 2021. iScience. 24: 102160. PMID: 33681726
- The c-Myc targeting hnRNPAB promotes lung adenocarcinoma cell proliferation via stabilization of CDK4 mRNA. | Xu, C., et al. 2023. Int J Biochem Cell Biol. 156: 106372. PMID: 36657708
- HnRNPAB is an independent prognostic factor in non‑small cell lung cancer and is involved in cell proliferation and metastasis. | Wang, Q., et al. 2023. Oncol Lett. 25: 215. PMID: 37153057
- hnRNPAB Promotes Pancreatic Ductal Adenocarcinoma Extravasation and Liver Metastasis by Stabilizing MYC mRNA. | Lei, K., et al. 2024. Mol Cancer Res. 22: 1022-1035. PMID: 38967522
- Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins. | Mayeda, A., et al. 1994. EMBO J. 13: 5483-95. PMID: 7957114
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
hnRNP A/B Antibody (G-12) | sc-390957 | 200 µg/ml | $316.00 | |||
hnRNP A/B Antibody (G-12): m-IgG Fc BP-HRP Bundle | sc-530314 | 200 µg Ab; 10 µg BP | $354.00 | |||
hnRNP A/B Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-523750 | 200 µg Ab, 40 µg BP | $354.00 | |||
hnRNP A/B Antibody (G-12) X | sc-390957 X | 200 µg/0.1 ml | $316.00 | |||
hnRNP A/B Antibody (G-12) AC | sc-390957 AC | 500 µg/ml, 25% agarose | $416.00 | |||
hnRNP A/B Antibody (G-12) HRP | sc-390957 HRP | 200 µg/ml | $316.00 | |||
hnRNP A/B Antibody (G-12) FITC | sc-390957 FITC | 200 µg/ml | $330.00 | |||
hnRNP A/B Antibody (G-12) PE | sc-390957 PE | 200 µg/ml | $343.00 | |||
hnRNP A/B Antibody (G-12) Alexa Fluor® 488 | sc-390957 AF488 | 200 µg/ml | $357.00 | |||
hnRNP A/B Antibody (G-12) Alexa Fluor® 546 | sc-390957 AF546 | 200 µg/ml | $357.00 | |||
hnRNP A/B Antibody (G-12) Alexa Fluor® 594 | sc-390957 AF594 | 200 µg/ml | $357.00 | |||
hnRNP A/B Antibody (G-12) Alexa Fluor® 647 | sc-390957 AF647 | 200 µg/ml | $357.00 | |||
hnRNP A/B Antibody (G-12) Alexa Fluor® 680 | sc-390957 AF680 | 200 µg/ml | $357.00 | |||
hnRNP A/B Antibody (G-12) Alexa Fluor® 790 | sc-390957 AF790 | 200 µg/ml | $357.00 |