HNP Antibody (H-2): sc-390796
- HNP Antibody (H-2) is a mouse monoclonal IgG1 κ HNP antibody provided at 200 µg/ml
- raised against amino acids 18-94 mapping at the C-terminus of HNP 3 of human origin
- HNP Antibody (H-2) is recommended for detection of precursor HNP-1 and HNP-3, and the mature cleavage product HNP-2 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-HNP Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HNP Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with HNP Antibody (H-2) (see ordering information below).
QUICK LINKS
SEE ALSO...
HNP Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects HNP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HNP Antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HNP protein, part of the defensin family, plays a crucial role in innate immune response, particularly in host defense against microbial infections. Defensins, including human neutrophil peptides (HNPs), are small cationic peptides produced predominantly by neutrophils and are essential for the body′s first line of defense. HNP-1, HNP-2, and HNP-3, which are secreted by neutrophils, constitute approximately 30% of total granule protein in these immune cells, highlighting their significance in combating pathogens. HNP-1 protein is encoded by the α1-defensin (DEFA1) gene, while HNP-3 is encoded by the α3-defensin (DEFA3) gene, and HNP-2 is a mature cleavage product of both precursor proteins. The presence of these peptides in neutrophil granules and their secretion during immune responses underscores their importance in phagocyte-mediated host defense, making anti-HNP antibody (H-2) a valuable tool for researchers studying immune responses and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HNP Antibody (H-2) References:
- Immunohistochemical staining of human alpha-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas. | Mizukawa, N., et al. 2000. Anticancer Res. 20: 1125-7. PMID: 10810407
- Contribution of human alpha-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor. | Zhang, L., et al. 2002. Science. 298: 995-1000. PMID: 12351674
- Antimicrobial activity of truncated alpha-defensin (human neutrophil peptide (HNP)-1) analogues without disulphide bridges. | Lundy, FT., et al. 2008. Mol Immunol. 45: 190-3. PMID: 17548109
- Crystal structure of defensin HNP-3, an amphiphilic dimer: mechanisms of membrane permeabilization. | Hill, CP., et al. 1991. Science. 251: 1481-5. PMID: 2006422
- Localization of the cryptdin locus on mouse chromosome 8. | Ouellette, AJ., et al. 1989. Genomics. 5: 233-9. PMID: 2571573
- Assignment of defensin gene(s) to human chromosome 8p23. | Sparkes, RS., et al. 1989. Genomics. 5: 240-4. PMID: 2793180
- Defensins. | Ganz, T. and Lehrer, RI. 1995. Pharmacol Ther. 66: 191-205. PMID: 7667395
- Inheritance of unequal numbers of the genes encoding the human neutrophil defensins HP-1 and HP-3. | Mars, WM., et al. 1995. J Biol Chem. 270: 30371-6. PMID: 8530462
- The human beta-defensin-1 and alpha-defensins are encoded by adjacent genes: two peptide families with differing disulfide topology share a common ancestry. | Liu, L., et al. 1997. Genomics. 43: 316-20. PMID: 9268634
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HNP Antibody (H-2) | sc-390796 | 200 µg/ml | $322.00 | |||
HNP Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-527650 | 200 µg Ab; 10 µg BP | $361.00 | |||
HNP Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-533023 | 200 µg Ab; 20 µg BP | $361.00 | |||
HNP Antibody (H-2) AC | sc-390796 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HNP Antibody (H-2) HRP | sc-390796 HRP | 200 µg/ml | $322.00 | |||
HNP Antibody (H-2) FITC | sc-390796 FITC | 200 µg/ml | $336.00 | |||
HNP Antibody (H-2) PE | sc-390796 PE | 200 µg/ml | $349.00 | |||
HNP Antibody (H-2) Alexa Fluor® 488 | sc-390796 AF488 | 200 µg/ml | $364.00 | |||
HNP Antibody (H-2) Alexa Fluor® 546 | sc-390796 AF546 | 200 µg/ml | $364.00 | |||
HNP Antibody (H-2) Alexa Fluor® 594 | sc-390796 AF594 | 200 µg/ml | $364.00 | |||
HNP Antibody (H-2) Alexa Fluor® 647 | sc-390796 AF647 | 200 µg/ml | $364.00 | |||
HNP Antibody (H-2) Alexa Fluor® 680 | sc-390796 AF680 | 200 µg/ml | $364.00 | |||
HNP Antibody (H-2) Alexa Fluor® 790 | sc-390796 AF790 | 200 µg/ml | $364.00 |