HNF-6 Antibody (B-1): sc-376308

HNF-6 Antibody (B-1) is a mouse monoclonal IgG2b antibody that detects HNF-6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HNF-6, a member of the hepatocyte nuclear factor family, plays a crucial role in the regulation of gene expression in various tissues, particularly in the liver, where HNF-6 is involved in the transcriptional activation of numerous hepatocyte-enriched genes. HNF-6 is primarily located in the nucleus, where HNF-6 binds to specific DNA sequences to modulate the transcription of target genes, which is essential for maintaining metabolic homeostasis and proper organ function. Notably, HNF-6 has been shown to interact with the promoter of HNF-3β, suggesting HNF-6′s involvement in gut endoderm epithelial cell differentiation, and HNF-6 may also act as a transcriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin. The ability of HNF-6 to regulate these critical genes underscores HNF-6′s importance in liver function and development, making anti-HNF-6 antibody (B-1) a valuable tool for researchers studying metabolic diseases and developmental biology.

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HNF-6 Antibody (B-1) References:

1. Involvement of STAT5 (signal transducer and activator of transcription 5) and HNF-4 (hepatocyte nuclear factor 4) in the transcriptional control of the hnf6 gene by growth hormone.  |  Lahuna, O., et al. 2000. Mol Endocrinol. 14: 285-94. PMID: 10674400
2. Maintaining HNF6 expression prevents AdHNF3beta-mediated decrease in hepatic levels of Glut-2 and glycogen.  |  Tan, Y., et al. 2002. Hepatology. 35: 790-8. PMID: 11915024
3. The onecut transcription factor HNF6 is required for normal development of the biliary tract.  |  Clotman, F., et al. 2002. Development. 129: 1819-28. PMID: 11934848
4. C/EBPalpha and HNF6 protein complex formation stimulates HNF6-dependent transcription by CBP coactivator recruitment in HepG2 cells.  |  Yoshida, Y., et al. 2006. Hepatology. 43: 276-86. PMID: 16440369
5. The hepatocyte nuclear factor 6 (HNF6) and FOXA2 are key regulators in colorectal liver metastases.  |  Lehner, F., et al. 2007. FASEB J. 21: 1445-62. PMID: 17283222
6. Orphan nuclear receptor SHP interacts with and represses hepatocyte nuclear factor-6 (HNF-6) transactivation.  |  Lee, YS., et al. 2008. Biochem J. 413: 559-69. PMID: 18459945
7. Multiple, temporal-specific roles for HNF6 in pancreatic endocrine and ductal differentiation.  |  Zhang, H., et al. 2009. Mech Dev. 126: 958-73. PMID: 19766716
8. The Onecut transcription factor HNF-6 contributes to proper reorganization of Purkinje cells during postnatal cerebellum development.  |  Audouard, E., et al. 2013. Mol Cell Neurosci. 56: 159-68. PMID: 23669529
9. Loss of HNF6 expression correlates with human pancreatic cancer progression.  |  Pekala, KR., et al. 2014. Lab Invest. 94: 517-27. PMID: 24638272
10. Growth Hormone Mediates Its Protective Effect in Hepatic Apoptosis through Hnf6.  |  Wang, K., et al. 2016. PLoS One. 11: e0167085. PMID: 27936029

The preferred HNF-6 antibody is HNF-6 Antibody (G-10): sc-376167.