Anti-HNF-6 Antibody (G-10) is a mouse monoclonal IgG1 (kappa light chain) HNF-6 antibody provided at 200 µg/ml
raised against amino acids 11-110 of HNF-6 of human origin
Anti-HNF-6 Antibody (G-10) is recommended for detection of HNF-6 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
Anti-HNF-6 Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for ChIP application (sc-376167 X, 200 µg/0.1 ml)
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HNF-6 (G-10): sc-376167.
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Labware ships FedEx Ground free of charge to the contiguous US
HNF-6 Antibody (G-10) is a high quality monoclonal HNF-6 antibody (also designated HNF6 antibody, ONECUT1 antibody or one cut homeobox 1 antibody) suitable for the detection of the HNF-6 protein of mouse, rat and human origin. HNF-6 Antibody (G-10) is available as both the non-conjugated anti-HNF-6 antibody form, as well as multiple conjugated forms of anti-HNF-6 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Are there any issues with using sc-376167: HNF-6 (G-10) mouse monoclonal primary antibody on mouse samples?
Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376167 conjugates.
Rated 5 out of
Perfect antibody for IF on human frozen sectionThis antibody gave a good staining result on frozen section of human retina.
Date published: 2019-05-22
Rated 4 out of
Works Good for IFHave tried with hepatoblasts and hepatocytes; shows good nuclear staining at 1:50 concentration
Date published: 2017-04-03
Rated 5 out of
Western blot analysis of HNFWestern blot analysis of HNF-6 expression in non-transfected: sc-117752 (A) and mouse HNF-6 transfected: sc-120850 (B) 293T whole cell lysates. -SCBT QC
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