HNF-3 beta Antibody (H-8) recommended for detection of HNF-3β of mouse, rat and human origin by WB, IP, IF and ELISA

HNF-3β Antibody (H-8): sc-271104

HNF-3β Antibody (H-8) is rated 5.0 out of 5 by 2.
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Datasheets

    • HNF-3β Antibody (H-8) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 261-370 mapping within an internal region of HNF-3β of human origin
    • recommended for detection of HNF-3β of mouse, rat and human origin by WB, IP, IF and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-271104 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HNF-3β (H-4): sc-374376 for HNF-3β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended fixation method for immunofluorescence staining with HNF-3β (H-8): sc-271104 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Fuzzy but strong bands seen in Western Blot Fuzzy but strong bands seen in Western Blot using Hep G2 and A549 nuclear extracts -SCBT QC
Date published: 2015-01-24
Rated 5 out of 5 by from Great for PFA fixed IF with Hep G2 cells Great for PFA fixed IF with Hep G2 cells -SCBT QC
Date published: 2013-09-22
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