HNF-3 beta Antibody (H-4) recommended for detection of HNF-3β of mouse, rat and human origin by WB, IP, IF and ELISA

HNF-3β Antibody (H-4): sc-374376

HNF-3β Antibody (H-4) is rated 4.5 out of 5 by 10.
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Datasheets

    • HNF-3β Antibody (H-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 431-459 at the C-terminus of HNF-3β of mouse origin
    • Anti-HNF-3 beta Antibody (H-4) is recommended for detection of HNF-3β of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-HNF-3 beta Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-374376 X, 200 µg/0.1 ml)
    • blocking peptide, sc-374376 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HNF-3β (H-4): sc-374376.
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HNF-3β抗体(H-4): sc-374376の無料試供品をいただきたいのですが,可能でしょうか?ワークすれば購入の予定です.

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2019-06-05

Hi, I plan on using this antibody for immunohistochemistry on floating brain tissue, would it be possible to get a sample of the antibody to test before we buy it? Thanks.

Asked by: Bondarenko
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

What application is the blocking peptide sc-374376 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good antibody Works as we expected. Tested by immunofluorescence. Great
Date published: 2018-12-18
Rated 5 out of 5 by from Nuclear localization in IF The sample of the antibody worked quite well for IF in a dilution of 1:200
Date published: 2018-01-24
Rated 3 out of 5 by from Works but not specific We bought this antibody to detect FOXA2 in prostate cancer cell lines. Based on published literature, FOXA2 protein is expressed in PC-3 cells but not in LNCaP cells. As the figure showed, the antibody works but can pick up non-specific bands in LNCaP cells.
Date published: 2017-12-15
Rated 4 out of 5 by from ICC on iPSC DA neuronal culture I tested the antibody for an ICC on iPSC DA neuronal culture, fixed 20min in paraformaldehyde 4%. Dilution: 1/100. It worked but there is a little bit of noise.
Date published: 2017-10-31
Rated 4 out of 5 by from Immunohistochemistry free floating I tested the antibody for an immunohistochemistry on mouse embryo spinal cord at E12.5, fixed 1h in paraformaldehyde 4%. Dilution: 1/100, Permeabilization solution: PBS 1X- Triton 0,2% - Goat serum 10%. It worked but there is a little bit of noise.
Date published: 2017-10-27
Rated 4 out of 5 by from gute Western Blotergebnisse WB mit Zelllysaten, Verdünnung 1:200, gute Signale mit ein wenig Backgroundsignal
Date published: 2017-10-18
Rated 5 out of 5 by from HNF-3beta/FOXA2 in neonatal rat brain Clear immunoreactivity in neonatal (PN2) rat brain, and absence of immunoreactivity in adult rat brain. Note only specific brain regions were examined. Used at 1:50, signal may be stronger with different dilutions/conditions.
Date published: 2017-08-22
Rated 5 out of 5 by from great features WB on mice Liver sample,diluted with 1:200,which shows good quality and got bands very clearly.
Date published: 2017-04-30
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