HNF-1 beta Antibody (94.8) recommended for detection of HNF-1β of human origin by WB and IP

HNF-1β Antibody (94.8): sc-130407

HNF-1β Antibody (94.8) is rated 4.0 out of 5 by 1.
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Datasheets

    • Anti-HNF-1β Antibody (94.8) is a mouse monoclonal IgG2a (kappa light chain) HNF-1β antibody provided at 100 µg/ml
    • raised against recombinant HNF-1β of human origin
    • recommended for detection of HNF-1β of human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HNF-1β (94.8): sc-130407.
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Hi, I am wondering if you also provide this antibody in an X/more concentrated format.

Asked by: bnch
Thank you for your question. Please contact our technical service directly.
Answered by: Tech Support Europe
Date published: 2019-09-23

Can this antibody (sc-130407) be used for mouse tissue? Can this antibody be used for immunofluorecence staining on paraffin sections?

Asked by: Xiangmin
Thank you for your question. This antibody has not been tested for mouse use, and is not recommended or warrantied for this use. We have not yet tested sc-130407 HNF-1β (94.8) on IHC. As a result, IHC is not currently a recommended application for this antibody.
Answered by: Tech Service
Date published: 2019-09-19

Is this antibody suitable for immunohistochemistry on formalin fixed paraffin embedded human tissue?

Asked by: cnotis
Thank you for your question. We have not yet tested this antibody on formalin-fixed paraffin embedded tissue. As a result, IHC(P) is not currently a recommended application for this antibody.
Answered by: Technical Support
Date published: 2018-12-13

Did you have any information on the effectiveness of this antibody on mouse liver IHC? Lineage fate of ductular reactions in carcinogenesis ... (Simone Jörs JCI 2015)

Asked by: MohamedBOUSALEH
Thank you for your question. This antibody sc-130407 is recommended for the detection of HNF-1 of human origin by WB and IP. We would not expect any significant cross-reactivity with mouse species. If you require more information, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Could I use this antibody for staining human ovary tumors in IHC? and if I could, how dilute range is recomended?

Asked by: a0061410
Thank you for your question. We have not yet tested this antibody, HNF-1β (94.8): sc-130407, for immunohistochemistry so we do not know if the antibody is suitable for this application. In general, we recommend using a starting dilution of 1:50 for IHC testing with similar antibodies.
Answered by: Technical Support
Date published: 2018-10-18

I am using HNF-1β (94.8): sc-130407 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24

Hi, I was wondering why the band showed in the western blot using HNF-1β Antibody sc-130407 is at 50 kDa and not higher around 60 kDa where I would expect based on the protein size.

Asked by: frdf
Thank you for your question. This antibody, HNF-1β Antibody (94.8): sc-130407, detected a signal around 50 kDa in human skin tissue extract by Western blotting. According to Uniprot, (http://www.uniprot.org/uniprot/P35680#sequences), HNF-1β is known to have several isoforms with predicted molecular weights of 61, 58, 45 and 47 kDa.
Answered by: Technical Support
Date published: 2017-03-14
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Rated 4 out of 5 by from Single band seen in Western Blot with human Single band seen in Western Blot with human skin tissue extract -SCBT QC
Date published: 2014-08-24
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