HNF-1 alpha Antibody (F-7) recommended for detection of HNF-1α of mouse, rat and human origin by WB, IP, IF and ELISA

HNF-1α Antibody (F-7): sc-393925

HNF-1α Antibody (F-7) is rated 4.3 out of 5 by 7.
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Datasheets

    • Anti-HNF-1α Antibody (F-7) is a mouse monoclonal IgG2a (kappa light chain) HNF-1α antibody provided at 200 µg/ml
    • raised against amino acids 80-284 of HNF-1α of human origin
    • Anti-HNF-1 alpha Antibody (F-7) is recommended for detection of HNF-1α of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
    • Anti-HNF-1 alpha Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-393925 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HNF-1α (F-7): sc-393925.
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Can HNF-1α (F-7): sc-393925 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HNF-1α (F-7): sc-393925 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 抗体不错 本人半月前申请的HNF-1a小样试用装,跑了蛋白,条带不错,推荐使用,另外,有现货的话,基本一天到货,赞
Date published: 2017-09-22
Rated 4 out of 5 by from Good The antibody works well, especially after overnight incubation. I used at 1:1000 dilution.The band is strong and clear,the background is also clear.
Date published: 2017-07-14
Rated 5 out of 5 by from Great has been the antibody, than we have to buy t Salesman to apply for HBF1a antibodies, used, and very good use of this protein before the Abcam has not been used to do the experiment, although it is a trial installation, but better than before.
Date published: 2017-01-07
Rated 1 out of 5 by from Unsuitable for IHC(P) Unsuitable for IHC(P). Unspecifically stains confirmed HNF1A negative samples at various dilutions, no difference between HNF1A positive and negative samples. Cytoplasmic instead of nuclear staining. Not an alternative for sc-8986 for this application.
Date published: 2016-08-16
Rated 5 out of 5 by from I used I used, it is good to use, than before to buy some of the antibodies are actually better use
Date published: 2016-08-02
Rated 5 out of 5 by from Very good quality on Rat Liver tissue in WB Very good quality on Rat Liver tissue in WB.we tried 1:500 to 1:100. The optimize dilution is 1:300. I use lot:B0514. I give full score of this antibody. I'll order again.
Date published: 2015-09-09
Rated 5 out of 5 by from Great results in Western Blot with Hep G2 Great results in Western Blot with Hep G2 whole cell lysate and human liver and rat liver tissue extracts -SCBT QC
Date published: 2014-02-15
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