HNF-1α Antibody (F-7): sc-393925

HNF-1α Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect hepatocyte nuclear factor 1-alpha (HNF-1α), also known as HNF1A or transcription factor 1 (TCF1). Anti-HNF-1α antibody (F-7) is raised against amino acids 80-284 of the human HNF-1α protein and demonstrates robust reactivity with HNF-1α from human, mouse, rat, canine, and porcine species, making it versatile for various experimental models. Validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), HNF-1α (F-7) antibody facilitates the investigation of this crucial nuclear transcription factor. Located within the cell nucleus, HNF-1α plays a pivotal role in regulating gene expression involved in liver development, metabolism, and the function of other organs such as the kidney and pancreas. HNF-1α importance is underscored by its association with several diseases, including diabetes, polycystic kidney disease, and certain cancers, highlighting its relevance in both basic research and clinical studies. Available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® derivatives, HNF-1α monoclonal antibody (F-7) offers flexibility for diverse experimental applications. By targeting HNF-1α, researchers can gain valuable insights into gene regulation mechanisms and disease pathways, making anti-HNF-1α antibody (F-7) an essential tool for advancing biomedical research.

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HNF-1α Antibody (F-7) References:

1. Common variants in HNF-1 alpha and risk of type 2 diabetes.  |  Holmkvist, J., et al. 2006. Diabetologia. 49: 2882-91. PMID: 17033837
2. Comparative analysis of retroviral and native promoters driving expression of beta1,3-galactosyltransferase beta3Gal-T5 in human and mouse tissues.  |  Mare, L. and Trinchera, M. 2007. J Biol Chem. 282: 49-57. PMID: 17107959
3. Renal cysts and diabetes due to a heterozygous HNF-1beta gene deletion.  |  Eller, P., et al. 2007. Nephrol Dial Transplant. 22: 1271-2. PMID: 17127696
4. Analysis of bile acid-induced regulation of FXR target genes in human liver slices.  |  Jung, D., et al. 2007. Liver Int. 27: 137-44. PMID: 17241392
5. Digenic inheritance of hepatocyte nuclear factor-1alpha and -1beta with maturity-onset diabetes of the young, polycystic thyroid, and urogenital malformations.  |  Karges, B., et al. 2007. Diabetes Care. 30: 1613-4. PMID: 17337496
6. More potent transcriptional activators or a transdominant inhibitor of the HNF1 homeoprotein family are generated by alternative RNA processing.  |  Bach, I. and Yaniv, M. 1993. EMBO J. 12: 4229-42. PMID: 7900999
7. The HNF-3 gene family of transcription factors in mice: gene structure, cDNA sequence, and mRNA distribution.  |  Kaestner, KH., et al. 1994. Genomics. 20: 377-85. PMID: 8034310
8. Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes.  |  Drewes, T., et al. 1996. Mol Cell Biol. 16: 925-31. PMID: 8622695
9. The transcriptional activator hepatocyte nuclear factor 6 regulates liver gene expression.  |  Samadani, U. and Costa, RH. 1996. Mol Cell Biol. 16: 6273-84. PMID: 8887657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HNF-1α Antibody (F-7) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-HNF-1α Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HNF-1α Antibody (F-7) Specifications

Alternate Product Name: HNF1A

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 80-284

Epitope Target: HNF-1α

Epitope Species: human

Product Reactivity/Detection: HNF-1α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for HNF-1α Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent