HMG-1/HMGB1 Antibody (W-18): sc-74085

HMG-1 Antibody (W-18) is a mouse monoclonal IgG2b antibody that detects HMG-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-HMG-1 antibody (W-18) is available in a non-conjugated form, allowing for versatile experimental use. High mobility group (HMG) proteins, including HMG-1, play a crucial role in regulating gene expression by altering DNA structure. HMG-1 binds to DNA and induces significant conformational changes, such as bending and unwinding of the double helix, which facilitates transcription factor binding and enhances transcriptional activity. This function is vital for cellular processes such as differentiation, development, and stress response. HMG-1 interacts with various proteins, including Oct-2 and members of the NFκB family, to synergistically activate transcription. Phosphorylation of HMG-1 is essential for transcriptional activity, highlighting the importance of post-translational modifications in regulating function. With an ability to bind single-stranded DNA and induce conformational changes in double-stranded DNA, HMG-1 is a key player in chromatin dynamics and gene regulation, making HMG-1 (W-18) monoclonal antibody an invaluable tool for researchers studying these critical biological processes.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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HMG-1/HMGB1 Antibody (W-18) References:

1. HMG-1 as a mediator of acute lung inflammation.  |  Abraham, E., et al. 2000. J Immunol. 165: 2950-4. PMID: 10975801
2. HMG1 and 2, and related 'architectural' DNA-binding proteins.  |  Thomas, JO. and Travers, AA. 2001. Trends Biochem Sci. 26: 167-74. PMID: 11246022
3. Identity of nuclear high-mobility-group protein, HMG-1, and sulfoglucuronyl carbohydrate-binding protein, SBP-1, in brain.  |  Chou, DK., et al. 2001. J Neurochem. 77: 120-31. PMID: 11279268
4. HMG-1 enhances HMG-I/Y binding to an A/T-rich enhancer element from the pea plastocyanin gene.  |  Webster, CI., et al. 2001. Eur J Biochem. 268: 3154-62. PMID: 11389716
5. The binding interaction of HMG-1 with the TATA-binding protein/TATA complex.  |  Das, D. and Scovell, WM. 2001. J Biol Chem. 276: 32597-605. PMID: 11390376
6. Mutations in hmg1, Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus.  |  Rybak, JM., et al. 2019. mBio. 10: PMID: 30940706
7. High mobility group protein, HMG-1, contains insignificant glycosyl modification.  |  Chao, YB., et al. 1994. Protein Sci. 3: 2452-4. PMID: 7757000
8. Competition between HMG-I(Y), HMG-1 and histone H1 on four-way junction DNA.  |  Hill, DA. and Reeves, R. 1997. Nucleic Acids Res. 25: 3523-31. PMID: 9254714
9. High mobility group protein-1 (HMG-1) is a unique activator of p53.  |  Jayaraman, L., et al. 1998. Genes Dev. 12: 462-72. PMID: 9472015
10. DNA-binding properties of the tandem HMG boxes of high-mobility-group protein 1 (HMG1).  |  Grasser, KD., et al. 1998. Eur J Biochem. 253: 787-95. PMID: 9654080

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HMG-1/HMGB1 Antibody (W-18) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-HMG-1/HMGB1 Antibody (W-18) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HMG-1/HMGB1 Antibody (W-18) Specifications

Alternate Product Name: HMG-1

Product Clone Name: W-18

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: HMG-1

Epitope Species: human

Product Reactivity/Detection: HMG-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 50 µg IgG_2b in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for HMG-1/HMGB1 Antibody (W-18): N/A

The preferred HMG-1/HMGB1 antibody is HMG-1/HMGB1 Antibody (D-3): sc-518191.