HMG-1/HMGB1 Antibody (J2E1): sc-135809

HMG-1 Antibody (J2E1) is a mouse monoclonal IgG2b antibody that detects HMG-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-HMG-1 antibody (J2E1) is available in a non-conjugated form, allowing for versatile experimental use. High mobility group (HMG) proteins, including HMG-1, play a crucial role in the regulation of gene expression by binding to DNA and inducing structural changes that facilitate transcription. Specifically, HMG-1 is known to interact with various transcription factors, such as Oct-2 and members of the NFκB family, enhancing their ability to activate transcription. This interaction is vital for cellular responses to stress and inflammation, as HMG-1 can also act as a signaling molecule outside the nucleus, influencing immune responses. Additionally, HMG-1′s two DNA-binding domains, known as HMG boxes, allow binding to single-stranded DNA and induce conformational changes in double-stranded DNA, which is essential for the proper functioning of many biological processes. The phosphorylation of HMG-1 is another important aspect, as phosphorylation is required to stimulate transcriptional activity, further underscoring HMG-1′s significance in cellular regulation and gene expression.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HMG-1/HMGB1 Antibody (J2E1) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-HMG-1/HMGB1 Antibody (J2E1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HMG-1/HMGB1 Antibody (J2E1) Specifications

Alternate Product Name: HMG-1

Product Clone Name: J2E1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 1-215

Epitope Target: HMG-1

Epitope Species: human

Product Reactivity/Detection: HMG-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 1% glycerol

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for HMG-1/HMGB1 Antibody (J2E1): N/A

The preferred HMG-1/HMGB1 antibody is HMG-1/HMGB1 Antibody (D-3): sc-518191.