HMG-1/HMGB1 Antibody (HAP46.5): sc-56698

HMG-1 Antibody (HAP46.5) is a mouse monoclonal IgG1 kappa light chain antibody that detects HMG-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-HMG-1 antibody (HAP46.5) is available as a non-conjugated format. The high mobility group (HMG) proteins, including HMG-1, play a crucial role in the regulation of gene expression and chromatin structure. Specifically, HMG-1 interacts with various transcription factors, such as Oct-2 and members of the NFκB family, enhancing their ability to activate transcription. This interaction is vital for cellular responses to stress and inflammation, as HMG-1 can act as a signaling molecule that influences the immune response. HMG-1 is predominantly located in the nucleus, where binding to DNA induces structural changes that facilitate the accessibility of transcription machinery to specific genes. The presence of two DNA-binding domains, known as HMG boxes, allows HMG-1 to bind to both single-stranded and double-stranded DNA, although primarily inducing conformational changes in double-stranded DNA. Understanding HMG-1 function and interactions is essential for elucidating its role in various biological processes, including development, differentiation, and cellular response to injury.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HMG-1/HMGB1 Antibody (HAP46.5) References:

1. HMG-1 as a late mediator of endotoxin lethality in mice.  |  Wang, H., et al. 1999. Science. 285: 248-51. PMID: 10398600
2. High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes.  |  Andersson, U., et al. 2000. J Exp Med. 192: 565-70. PMID: 10952726
3. HMG-1 rediscovered as a cytokine.  |  Yang, H., et al. 2001. Shock. 15: 247-53. PMID: 11303722
4. Structure of a gene coding for human HMG2 protein.  |  Shirakawa, H. and Yoshida, M. 1992. J Biol Chem. 267: 6641-5. PMID: 1551873
5. Structural features of the HMG chromosomal proteins and their genes.  |  Bustin, M., et al. 1990. Biochim Biophys Acta. 1049: 231-43. PMID: 2200521
6. A human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1.  |  Wen, L., et al. 1989. Nucleic Acids Res. 17: 1197-214. PMID: 2922262
7. A secondary mechanism of action for triazole antifungals in Aspergillus fumigatus mediated by hmg1.  |  Rybak, JM., et al. 2024. Nat Commun. 15: 3642. PMID: 38684680
8. HMGI(Y) and Sp1 in addition to NF-kappa B regulate transcription of the MGSA/GRO alpha gene.  |  Wood, LD., et al. 1995. Nucleic Acids Res. 23: 4210-9. PMID: 7479086
9. Interleukin 4-inducible phosphorylation of HMG-I(Y) is inhibited by rapamycin.  |  Wang, DZ., et al. 1995. J Biol Chem. 270: 22924-32. PMID: 7559428
10. Structural basis for DNA bending by the architectural transcription factor LEF-1.  |  Love, JJ., et al. 1995. Nature. 376: 791-5. PMID: 7651541
11. Changes in superhelicity are introduced into closed circular DNA by binding of high mobility group protein I/Y.  |  Nissen, MS. and Reeves, R. 1995. J Biol Chem. 270: 4355-60. PMID: 7876198
12. Reversal of intrinsic DNA bends in the IFN beta gene enhancer by transcription factors and the architectural protein HMG I(Y).  |  Falvo, JV., et al. 1995. Cell. 83: 1101-11. PMID: 8548798

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HMG-1/HMGB1 Antibody (HAP46.5) has 59 citations in a variety of scientific publications.

Primary antibodies like Anti-HMG-1/HMGB1 Antibody (HAP46.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HMG-1/HMGB1 Antibody (HAP46.5) Specifications

Alternate Product Name: HMG-1

Product Clone Name: HAP46.5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: HMG-1

Epitope Species: human

Product Reactivity/Detection: HMG-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: Arabadopsis

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 59 citations in various scientific publications

Available Conjugates/Forms for HMG-1/HMGB1 Antibody (HAP46.5): N/A

The preferred HMG-1/HMGB1 antibody is HMG-1/HMGB1 Antibody (D-3): sc-518191.