HLA-G Antibody (4H84): sc-21799
- HLA-G Antibody (4H84) is a mouse monoclonal IgG1 κ HLA-G antibody, cited in 83 publications, provided at 200 µg/ml
- raised against amino acids 61-83 of HLA-G of human origin
- HLA-G Antibody (4H84) is recommended for detection of HLA-G isoforms of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-HLA-G Antibody (4H84) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HLA-G Antibody (4H84) for WB and IHC(P) applications. These reagents are now offered in bundles with HLA-G Antibody (4H84) (see ordering information below).
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HLA-G Antibody (4H84) is a mouse monoclonal IgG1 kappa light chain antibody that detects HLA-G protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-HLA-G antibody (4H84) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HLA-G plays a crucial role in immune tolerance, particularly during pregnancy, by inhibiting the activation of maternal T lymphocytes and preventing fetal rejection. This unique function is vital for maintaining a healthy pregnancy, as HLA-G allows the semi-allogeneic fetus to evade the maternal immune response. HLA-G is primarily expressed in trophoblast cells of the placenta, where HLA-G acts as a protective barrier, ensuring that the developing fetus is not attacked by the mother′s immune system. HLA-G′s ability to modulate immune responses is attributed to interaction with inhibitory receptors on immune cells, such as the leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1) and the killer immunoglobulin-like receptor (KIR) family, which further underscores importance in both reproductive immunology and potential therapeutic applications in transplant medicine and cancer immunotherapy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HLA-G Antibody (4H84) References:
- Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. | Fournel, S., et al. 2000. Tissue Antigens. 55: 510-8. PMID: 10902607
- HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. | Fuzzi, B., et al. 2002. Eur J Immunol. 32: 311-5. PMID: 11807769
- Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases. | Pangault, C., et al. 2002. Hum Immunol. 63: 83-90. PMID: 11821155
- Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. | Lozano, JM., et al. 2002. AIDS. 16: 347-51. PMID: 11834945
- Disulfide bond-mediated dimerization of HLA-G on the cell surface. | Boyson, JE., et al. 2002. Proc Natl Acad Sci U S A. 99: 16180-5. PMID: 12454284
- Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. | Menier, C., et al. 2003. Hum Immunol. 64: 315-26. PMID: 12590976
- The many faces of human leukocyte antigen-G: relevance to the fate of pregnancy. | Dahl, M., et al. 2014. J Immunol Res. 2014: 591489. PMID: 24741608
- Plasma human leukocyte antigen-G (HLA-G) in patients with thyroid cancer. | Akın, M., et al. 2017. Turk J Med Sci. 47: 1263-1266. PMID: 29156872
- Prognostic and Clinicopathological Value of Human Leukocyte Antigen G in Gastrointestinal Cancers: A Meta-Analysis. | Peng, Y., et al. 2021. Front Oncol. 11: 642902. PMID: 34055611
- Human leukocyte antigen-G (HLA-G) expression plays an important role in the diagnosis and grading of endometrial cancer. | Bai, Y., et al. 2022. J Obstet Gynaecol. 42: 641-647. PMID: 34382498
- HLA-G and Recurrent Pregnancy Loss. | Barbaro, G., et al. 2023. Int J Mol Sci. 24: PMID: 36768880
- Human leukocyte antigen-G in gynaecological tumours. | Guo, X., et al. 2023. Int J Immunogenet. 50: 163-176. PMID: 37415092
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-G Antibody (4H84) | sc-21799 | 200 µg/ml | $322.00 | |||
HLA-G Antibody (4H84): m-IgG Fc BP-HRP Bundle | sc-528372 | 200 µg Ab; 10 µg BP | $361.00 | |||
HLA-G Antibody (4H84): m-IgGκ BP-HRP Bundle | sc-520734 | 200 µg Ab, 40 µg BP | $361.00 | |||
HLA-G Antibody (4H84): m-IgG1 BP-HRP Bundle | sc-542900 | 200 µg Ab; 20 µg BP | $361.00 | |||
HLA-G Antibody (4H84) AC | sc-21799 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HLA-G Antibody (4H84) HRP | sc-21799 HRP | 200 µg/ml | $322.00 | |||
HLA-G Antibody (4H84) FITC | sc-21799 FITC | 200 µg/ml | $336.00 | |||
HLA-G Antibody (4H84) PE | sc-21799 PE | 200 µg/ml | $349.00 | |||
HLA-G Antibody (4H84) Alexa Fluor® 488 | sc-21799 AF488 | 200 µg/ml | $364.00 | |||
HLA-G Antibody (4H84) Alexa Fluor® 546 | sc-21799 AF546 | 200 µg/ml | $364.00 | |||
HLA-G Antibody (4H84) Alexa Fluor® 594 | sc-21799 AF594 | 200 µg/ml | $364.00 | |||
HLA-G Antibody (4H84) Alexa Fluor® 647 | sc-21799 AF647 | 200 µg/ml | $364.00 | |||
HLA-G Antibody (4H84) Alexa Fluor® 680 | sc-21799 AF680 | 200 µg/ml | $364.00 | |||
HLA-G Antibody (4H84) Alexa Fluor® 790 | sc-21799 AF790 | 200 µg/ml | $364.00 |