HLA-DRα Antibody (DA6.147): sc-53499

HLA-DRα Antibody (DA6.147) is a mouse monoclonal IgG1 antibody that detects HLA-DRα in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). HLA-DRα is a crucial component of the major histocompatibility complex (MHC) class II molecules, which play a vital role in the immune response by presenting processed antigens to CD4+ T helper cells. Proper functioning of HLA-DRα is essential for the activation of the adaptive immune system, as HLA-DRα facilitates the recognition of foreign antigens, thereby influencing the development of immune responses. Located primarily on the surface of antigen-presenting cells, including B cells and dendritic cells, HLA-DRα′s presence in these locations is critical for initiating T cell-mediated immunity. Furthermore, HLA-DRα interaction with various peptide antigens is regulated by other MHC molecules, such as HLA-DM and HLA-DO, which ensure that only appropriate peptides are presented to T cells. Understanding HLA-DRα dynamics and interactions is important for elucidating mechanisms of immune regulation and potential implications in autoimmune diseases, where dysregulation of antigen presentation can lead to inappropriate immune responses. Anti-HLA-DRα antibody (DA6.147) is an invaluable tool for researchers studying the complexities of immune function and the role of MHC class II molecules in health and disease.

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HLA-DRα Antibody (DA6.147) References:

1. [Construction and expression of HLA-DRalpha, DRB1*0401 transgenic mice model].  |  Deng, J., et al. 1999. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 16: 360-3. PMID: 10581343
2. An ATF/CREB binding motif is required for aberrant constitutive expression of the MHC class II DR alpha promoter and activation by SV40 T-antigen.  |  Cox, PM. and Goding, CR. 1992. Nucleic Acids Res. 20: 4881-7. PMID: 1329030
3. A new monoclonal antibody recognizing a linear determinant on the HLA-DRalpha chain N-terminus.  |  Ting, YT., et al. 2009. Hybridoma (Larchmt). 28: 423-9. PMID: 20025501
4. [Molecular cloning and three-dimensional structure modelling of HLA-DRalpha * 0101, DRbeta1 * 04051 and DRbeta1 * 07011].  |  Sun, YY., et al. 2001. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 17: 281-4. PMID: 21189634
5. Molecular cytogenetic analysis of human breast tumors: methylation pattern of the HLA-DRα gene.  |  Barbieri, R., et al. 1987. Cytotechnology. 1: 83-5. PMID: 22358447
6. Major Histocompatibility Complex Class II HLA-DRα Is Downregulated by Kaposi's Sarcoma-Associated Herpesvirus-Encoded Lytic Transactivator RTA and MARCH8.  |  Sun, Z., et al. 2016. J Virol. 90: 8047-58. PMID: 27356905
7. Significance of incorporation of DNMT1 and HLA-DRα with TNM staging in patients with hepatocellular carcinoma after curative resection.  |  Tang, H., et al. 2017. Int J Clin Exp Pathol. 10: 9372-9381. PMID: 31966809
8. The HLA-DQ(alpha 1*0102, beta 1*0602) heterodimer may confer susceptibility to multiple sclerosis in the absence of the HLA-DR(alpha 1*01, beta 1*1501) heterodimer.  |  Spurkland, A., et al. 1997. Tissue Antigens. 50: 15-22. PMID: 9243750
9. Novel glycosylation of HLA-DRalpha disrupts antigen presentation without altering endosomal localization.  |  Guerra, CB., et al. 1998. J Immunol. 160: 4289-97. PMID: 9574531
10. Regulation of HLA-DR and costimulatory B7 molecules in human thyroid carcinoma cells: differential binding of transcription factors to the HLA-DRalpha promoter.  |  Lahat, N., et al. 1998. Thyroid. 8: 361-9. PMID: 9623725

The preferred HLA-DRα antibody is HLA-DRα Antibody (G-7): sc-55593.