Date published: 2025-10-21

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Histone H3 Antibody (1G1): sc-517576

4.7(10)
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Datasheets
  • Histone H3 Antibody (1G1) is a mouse monoclonal IgG, cited in 87 publications, provided at 100 µg/ml
  • raised against recombinant Histone H3 protein fragments of human origin
  • recommended for detection of Histone H3 of mouse, rat and human origin by WB and IF
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Histone H3 Antibody (1G1) for WB applications. These reagents are now offered in bundles with Histone H3 Antibody (1G1) (see ordering information below).

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Histone H3 Antibody (1G1) is a mouse monoclonal IgG antibody that detects Histone H3 in mouse, rat, and human samples by western blotting (WB) and immunofluorescence (IF). Anti-Histone H3 antibody (1G1) is available in non-conjugated form. Histone H3 plays a crucial role in chromatin′s structural organization, essential for DNA packaging within the nucleus. Histone H3 forms a core part of the nucleosome′s octameric structure that wraps DNA, regulating gene expression and DNA replication. Histone H3 maintains genomic stability and facilitates transcription and repair processes. Histone H3 undergoes various post-translational modifications, including methylation and acetylation, influencing DNA and protein interactions, affecting chromatin structure and function. These modifications regulate gene expression and impact cellular differentiation and environmental response. Anti-Histone H3 antibody (1G1) detection capabilities make this reagent valuable for studying chromatin dynamics and epigenetic regulation in various biological contexts.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Histone H3 Antibody (1G1)

sc-517576
100 µg/ml
$316.00

Histone H3 Antibody (1G1): m-IgG Fc BP-HRP Bundle

sc-541115
100 µg Ab; 10 µg BP
$354.00

Histone H3 Antibody (1G1): m-IgG1 BP-HRP Bundle

sc-542591
100 µg Ab; 20 µg BP
$354.00

I performed ICC with this antibody in mitotic cells, but I couldn't detect histone H3 signal co-localized with the DAPI signal. Could you help me solve this issue?

Asked by: KWJ94
Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. There are many possible causes for no signal. Please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website, and we would be happy to help you figure this out. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase and will do our best to help you achieve the best results possible!
Answered by: Jiajun Z
Date published: 2024-09-29

Hi can we please get a sample of sc-517576? Do you have that available? We just purchased sc-390935 with Texas A&M University Biology Department, Dr. Deb Bell-Pedersen lab.

Asked by: Johnny
Thank you for contacting Santa Cruz Biotechnology. To request a sample please email scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-06-18

Just received a free sample, which dilution would you recommend for nuclear extracts WB?

Asked by: IngOlmos
Thank you for your question. Dilution recommendations can be found in the product's datasheet, which is available right here on the product page. For western blots we recommend a starting dilution of 1:200, and a dilution range between 1:100-1:1000. When working with nuclear extracts, an amount of protein between 10–20 µg is appropriate.
Answered by: Tech Support Europe
Date published: 2023-05-15

could it be possible to buy directly bound to alexa488 or 594 please

Asked by: antonio blesa
Thank you for your question. If you are interested in this antibody directly conjugated with AF488 or AF594, please contact our our Technical Service. We should be able to provide this format as special order.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-31
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Rated 4 out of 5 by from Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2023-02-17
Rated 5 out of 5 by from H3 Ab for Western BlottingAsked for a sample before purchasing. Worked straight away on CRC cell lines HCT116 and DLD1 (drug treated and untreated) when loading 20 ug of whole protein lysates and using 1:500 dilution in 5% BSA/TBST. Would definitely recommend.
Date published: 2023-01-24
Rated 5 out of 5 by from western blotI used this antibody for my mouse primary neuron cells western blot. I got very nice and clean bolt!
Date published: 2022-08-24
Rated 5 out of 5 by from good and strongThe antibody was tested on whole lysate from liver cancer cell lines (JHH6 andHuh7) and immortalized hepatocytes (IHH). Antibody: sensitive and specific. Too strong signal with the dilution you recommend. Conditions need to be better set. Apparent MW: about 15kDa. Expected MW: 15kDa. • 40ug/lane cell lysate • Transfer 1h30 100V, PVDF (0.2 µm) • Blocking milk 4% in 0.1% Tween -PBS 1h RT • Anti-HISTONE H3 (1G1)(sc-517576) 1:200 O/N 4°C in blocking solution • Anti-mouse HRP 1:1000 1h RT in blocking solution • Developed with ECL Plus
Date published: 2022-06-09
Rated 5 out of 5 by from Very good!In MB231 cells, a clear band appeared after WB analysis 1: 2000 dilution.
Date published: 2022-03-18
Rated 4 out of 5 by from Useful for my purposesI asked it as free trial and i worked well enough, so I think I can use it for my purposes.
Date published: 2020-12-23
Rated 5 out of 5 by from Great for IFBought this antibody recently for the purpose of immunofluorescence on human neuroblastoma cell lines. It worked very well.
Date published: 2019-09-03
Rated 5 out of 5 by from Works very well with 1:5000 dilutionWorks very well with 1:5000 dilution, and I hope some more conjugation products can be developed with this antibody.
Date published: 2019-02-05
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Histone H3 Antibody (1G1) is rated 4.7 out of 5 by 10.
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