HIPK2 Antibody (F-189): sc-100383

HIPK2 Antibody (F-189) is a mouse monoclonal IgG2a kappa light chain antibody that detects HIPK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). HIPK2 (F-189) antibody is available as a non-conjugated format. Homeodomain-Interacting Protein Kinase 2 (HIPK2) is a crucial member of the HIPK family, which includes HIPK1 and HIPK3, and is characterized by a conserved protein kinase domain that interacts with homeoproteins. HIPK2 functions as a co-repressor of homeodomain transcription factors, influencing gene expression and developmental pathways. HIPK2 regulates apoptosis and cellular stress responses, maintaining cellular homeostasis. HIPK2 localizes primarily in the nucleus, participating in nuclear body formation involved in gene expression regulation and stress response. HIPK2 presence in nuclear structures underscores a significant role in cellular signaling pathways and potential involvement in cancer and autoimmunity. HIPK2 undergoes SUMO-1 conjugation, enhancing stability and function while directing nuclear body localization crucial for stress and viral infection responses. Such modification influences protein interactions and cellular activity.

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HIPK2 Antibody (F-189) References:

1. The nuclear dot protein sp100, characterization of domains necessary for dimerization, subcellular localization, and modification by small ubiquitin-like modifiers.  |  Sternsdorf, T., et al. 1999. J Biol Chem. 274: 12555-66. PMID: 10212234
2. Viral immediate-early proteins abrogate the modification by SUMO-1 of PML and Sp100 proteins, correlating with nuclear body disruption.  |  Müller, S. and Dejean, A. 1999. J Virol. 73: 5137-43. PMID: 10233977
3. PML is critical for ND10 formation and recruits the PML-interacting protein daxx to this nuclear structure when modified by SUMO-1.  |  Ishov, AM., et al. 1999. J Cell Biol. 147: 221-34. PMID: 10525530
4. Covalent modification of the homeodomain-interacting protein kinase 2 (HIPK2) by the ubiquitin-like protein SUMO-1.  |  Kim, YH., et al. 1999. Proc Natl Acad Sci U S A. 96: 12350-5. PMID: 10535925
5. The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme.  |  Schwarz, SE., et al. 1998. Proc Natl Acad Sci U S A. 95: 560-4. PMID: 9435231
6. Homeodomain-interacting protein kinases, a novel family of co-repressors for homeodomain transcription factors.  |  Kim, YH., et al. 1998. J Biol Chem. 273: 25875-9. PMID: 9748262

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HIPK2 Antibody (F-189) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-HIPK2 Antibody (F-189) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HIPK2 Antibody (F-189) Specifications

Alternate Product Name: Homeodomain Interacting Protein Kinase 2

Product Clone Name: F-189

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 961-1065

Epitope Target: HIPK2

Epitope Species: human

Product Reactivity/Detection: HIPK2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for HIPK2 Antibody (F-189): N/A