HIF PHD3 Antibody (EG188e/d5): sc-517601

HIF PHD3 Antibody (EG188e/d5) is a mouse monoclonal IgG1 kappa light chain antibody that detects the HIF PHD3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-HIF PHD3 antibody (EG188e/d5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIF PHD3, also known as Egl-9 Family Hypoxia Inducible Factor 3, plays a crucial role in regulating cellular responses to hypoxia by hydroxylating hypoxia-inducible factor (HIF) α subunits, which are essential for transcriptional activation of genes involved in oxygen homeostasis. This post-translational modification determines the stability and activity of HIF-α proteins, thereby influencing processes such as angiogenesis, erythropoiesis, and metabolic adaptation to low oxygen levels. HIF PHD3 is widely expressed, with particularly high levels found in the placenta and heart, suggesting importance in developmental and cardiovascular processes. Understanding HIF PHD3 and its interactions with other proteins in the hypoxia signaling pathway can provide insights into various physiological and pathological conditions, including cancer and cardiovascular diseases.

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HIF PHD3 Antibody (EG188e/d5) References:

1. Hypoxia-inducible factor-1 (HIF-1) promotes its degradation by induction of HIF-alpha-prolyl-4-hydroxylases.  |  Marxsen, JH., et al. 2004. Biochem J. 381: 761-7. PMID: 15104534
2. Regulation of HIF prolyl hydroxylases by hypoxia-inducible factors.  |  Aprelikova, O., et al. 2004. J Cell Biochem. 92: 491-501. PMID: 15156561
3. Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.  |  Appelhoff, RJ., et al. 2004. J Biol Chem. 279: 38458-65. PMID: 15247232
4. Regulation of the prolyl hydroxylase domain protein 2 (phd2/egln-1) gene: identification of a functional hypoxia-responsive element.  |  Metzen, E., et al. 2005. Biochem J. 387: 711-7. PMID: 15563275
5. Potential regulation of glioma through the induction of apoptosis signaling via Egl-9 family hypoxia-inducible factor 3.  |  Mao, K., et al. 2017. Oncol Lett. 13: 893-897. PMID: 28356975
6. Hypoxia-inducible factor (HIF)-prolyl hydroxylase 3 (PHD3) maintains high HIF2A mRNA levels in clear cell renal cell carcinoma.  |  Miikkulainen, P., et al. 2019. J Biol Chem. 294: 3760-3771. PMID: 30617181
7. Esc-sEVs Rejuvenate Aging Hippocampal NSCs by Transferring SMADs to Regulate the MYT1-Egln3-Sirt1 Axis.  |  Hu, G., et al. 2021. Mol Ther. 29: 103-120. PMID: 33038325
8. Genome-Wide Knockout Screen Identifies EGLN3 Involving in Ammonia Neurotoxicity.  |  Li, J., et al. 2022. Front Cell Dev Biol. 10: 820692. PMID: 35425766
9. EGLN3 attenuates gastric cancer cell malignant characteristics by inhibiting JMJD8/NF-κB signalling activation independent of hydroxylase activity.  |  Cai, F., et al. 2024. Br J Cancer. 130: 597-612. PMID: 38184692
10. A Free Amino Acid Diet Alleviates Colorectal Tumorigenesis through Modulating Gut Microbiota and Metabolites.  |  Yu, YM., et al. 2024. Nutrients. 16: PMID: 38613073
11. High expression levels of haem oxygenase-1 promote ferroptosis in macrophage-derived foam cells and exacerbate plaque instability.  |  Guo, Z., et al. 2024. Redox Biol. 76: 103345. PMID: 39255694

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HIF PHD3 Antibody (EG188e/d5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-HIF PHD3 Antibody (EG188e/d5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HIF PHD3 Antibody (EG188e/d5) Specifications

Alternate Product Name: EGLN3

Product Clone Name: EG188e/d5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-100

Epitope Target: HIF PHD3

Epitope Species: human

Product Reactivity/Detection: HIF PHD3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for HIF PHD3 Antibody (EG188e/d5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin