HIF PHD2 Antibody (H-8): sc-271835
- HIF PHD2 Antibody (H-8) is a mouse monoclonal IgG1 κ HIF PHD2 antibody, cited in 20 publications, provided at 200 µg/ml
- raised against amino acids 240-288 mapping within an internal region of HIF PHD2 of human origin
- recommended for detection of HIF PHD2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-HIF PHD2 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HIF PHD2 Antibody (H-8) for WB applications. These reagents are now offered in bundles with HIF PHD2 Antibody (H-8) (see ordering information below).
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HIF PHD2 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HIF PHD2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HIF PHD2 monoclonal antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HIF PHD2 functions as a prolyl hydroxylase, catalyzing post-translational modification of hypoxia-inducible factor (HIF) alpha subunits, which regulates gene expression in response to oxygen levels. This modification determines HIF-α protein stability and activity, influencing cellular responses to hypoxia. HIF PHD2 shows predominant expression in skeletal muscle, heart, kidney, and brain, serving as a key regulator of oxygen homeostasis. HIF PHD2′s ability to hydroxylate HIF-α subunits enables cellular adaptation to varying oxygen conditions, making HIF PHD2 essential for angiogenesis, metabolism, and cell survival. HIF PHD2′s role in stabilizing HIF-1α under normoxic conditions maintains cellular function and environmental response capabilities.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HIF PHD2 Antibody (H-8) References:
- Hypoxia-inducible factor-1 (HIF-1) promotes its degradation by induction of HIF-alpha-prolyl-4-hydroxylases. | Marxsen, JH., et al. 2004. Biochem J. 381: 761-7. PMID: 15104534
- Regulation of HIF prolyl hydroxylases by hypoxia-inducible factors. | Aprelikova, O., et al. 2004. J Cell Biochem. 92: 491-501. PMID: 15156561
- Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor. | Appelhoff, RJ., et al. 2004. J Biol Chem. 279: 38458-65. PMID: 15247232
- Regulation of the prolyl hydroxylase domain protein 2 (phd2/egln-1) gene: identification of a functional hypoxia-responsive element. | Metzen, E., et al. 2005. Biochem J. 387: 711-7. PMID: 15563275
- HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells. | Fujita, N., et al. 2014. FASEB J. 28: 2455-65. PMID: 24558194
- Class I and IIa HDACs Mediate HIF-1α Stability Through PHD2-Dependent Mechanism, While HDAC6, a Class IIb Member, Promotes HIF-1α Transcriptional Activity in Nucleus Pulposus Cells of the Intervertebral Disc. | Schoepflin, ZR., et al. 2016. J Bone Miner Res. 31: 1287-99. PMID: 26765925
- Hepatic PHD2/HIF-1α axis is involved in postexercise systemic energy homeostasis. | Luo, B., et al. 2018. FASEB J. 32: 4670-4680. PMID: 29601782
- LRP5 Regulates HIF-1α Stability via Interaction with PHD2 in Ischemic Myocardium. | Ju, S., et al. 2021. Int J Mol Sci. 22: PMID: 34205318
- Adipose-Derived Mesenchymal Stem Cells Reprogram M1 Macrophage Metabolism via PHD2/HIF-1α Pathway in Colitis Mice. | Yuan, Y., et al. 2022. Front Immunol. 13: 859806. PMID: 35757749
- Adipocyte-derived lactate is a signalling metabolite that potentiates adipose macrophage inflammation via targeting PHD2. | Feng, T., et al. 2022. Nat Commun. 13: 5208. PMID: 36064857
- ETV2 regulating PHD2-HIF-1α axis controls metabolism reprogramming promotes vascularized bone regeneration. | Du, H., et al. 2024. Bioact Mater. 37: 222-238. PMID: 38549772
- Araloside A alleviates sepsis-induced acute lung injury via PHD2/HIF-1α in macrophages. | Ma, J., et al. 2024. Phytomedicine. 135: 156089. PMID: 39366158
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIF PHD2 Antibody (H-8) | sc-271835 | 200 µg/ml | $322.00 | |||
HIF PHD2 Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-527297 | 200 µg Ab; 10 µg BP | $361.00 | |||
HIF PHD2 Antibody (H-8): m-IgG1 BP-HRP Bundle | sc-532670 | 200 µg Ab; 20 µg BP | $361.00 | |||
HIF PHD2 Antibody (H-8) AC | sc-271835 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HIF PHD2 Antibody (H-8) HRP | sc-271835 HRP | 200 µg/ml | $322.00 | |||
HIF PHD2 Antibody (H-8) FITC | sc-271835 FITC | 200 µg/ml | $336.00 | |||
HIF PHD2 Antibody (H-8) PE | sc-271835 PE | 200 µg/ml | $349.00 | |||
HIF PHD2 Antibody (H-8) Alexa Fluor® 488 | sc-271835 AF488 | 200 µg/ml | $364.00 | |||
HIF PHD2 Antibody (H-8) Alexa Fluor® 546 | sc-271835 AF546 | 200 µg/ml | $364.00 | |||
HIF PHD2 Antibody (H-8) Alexa Fluor® 594 | sc-271835 AF594 | 200 µg/ml | $364.00 | |||
HIF PHD2 Antibody (H-8) Alexa Fluor® 647 | sc-271835 AF647 | 200 µg/ml | $364.00 | |||
HIF PHD2 Antibody (H-8) Alexa Fluor® 680 | sc-271835 AF680 | 200 µg/ml | $364.00 | |||
HIF PHD2 Antibody (H-8) Alexa Fluor® 790 | sc-271835 AF790 | 200 µg/ml | $364.00 |