HIF PHD1 Antibody (2G3): sc-293220
- HIF PHD1 Antibody (2G3) is a mouse monoclonal IgG2a κ HIF PHD1 antibody, cited in 1 publications, provided at 100 µg/ml
- raised against amino acids 1-407 representing full length HIF PHD1 of human origin
- recommended for detection of HIF PHD1 of human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HIF PHD1 Antibody (2G3). This work is in progress.
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HIF PHD1 Antibody (2G3) is a mouse monoclonal IgG2a kappa light chain antibody that detects the HIF PHD1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-HIF PHD1 antibody (2G3) is available as a non-conjugated format. HIF PHD1 (2G3) monoclonal antibody recognizes a member of the prolyl hydroxylase domain family that plays a crucial role in cellular oxygen sensing and regulation of gene expression in response to hypoxia. HIF PHD1 catalyzes the hydroxylation of proline residues in hypoxia-inducible factor (HIF) alpha subunits, essential for HIF degradation under normoxic conditions. This post-translational modification determines the stability and activity of HIF, a key transcription factor involved in cellular adaptation to low oxygen levels. HIF PHD1 shows predominant expression in testis and appears in various hormone-responsive tissues, including normal and cancerous mammary, ovarian, and prostate epithelium, highlighting significance in both physiological and pathological processes. EGLN2, the gene encoding HIF PHD1, is located on chromosome 19q13.3, with tightly regulated expression that influences cellular responses to oxygen availability.
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HIF PHD1 Antibody (2G3) References:
- Hypoxia-inducible factor-1 (HIF-1) promotes its degradation by induction of HIF-alpha-prolyl-4-hydroxylases. | Marxsen, JH., et al. 2004. Biochem J. 381: 761-7. PMID: 15104534
- Regulation of HIF prolyl hydroxylases by hypoxia-inducible factors. | Aprelikova, O., et al. 2004. J Cell Biochem. 92: 491-501. PMID: 15156561
- Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor. | Appelhoff, RJ., et al. 2004. J Biol Chem. 279: 38458-65. PMID: 15247232
- Regulation of the prolyl hydroxylase domain protein 2 (phd2/egln-1) gene: identification of a functional hypoxia-responsive element. | Metzen, E., et al. 2005. Biochem J. 387: 711-7. PMID: 15563275
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIF PHD1 Antibody (2G3) | sc-293220 | 100 µg/ml | $322.00 |