Hic-5 Antibody (H-1): sc-271447
- Hic-5 Antibody (H-1) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 409-434 near the C-terminus of Hic-5 of human origin
- recommended for detection of Hic-5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- TransCruz reagent for ChIP application (sc-271447 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Hic-5 Antibody (H-1) for WB applications. These reagents are now offered in bundles with Hic-5 Antibody (H-1) (see ordering information below).
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Hic-5 Antibody (H-1) is a mouse monoclonal IgG1 antibody that detects Hic-5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hic-5, also known as transforming factor beta 1 induced transcript 1, plays a crucial role in cellular signaling and adhesion through regulation of focal adhesion dynamics. Hic-5 contains four LIM motifs and seven zinc finger domains, which facilitate localization to the nuclear matrix and focal adhesion complexes. The LIM domains are essential for mediating interactions with focal adhesions, allowing anti-Hic-5 antibody (H-1) to inhibit integrin-mediated cell spreading on fibronectin by competing with paxillin for binding to focal adhesion kinase, thus preventing downstream signal transduction. This regulatory function is particularly important as increased expression of Hic-5 can lead to cellular senescence in developing fibroblasts, while during myogenesis, Hic-5 blocks differentiation and induces apoptosis in developing myoblasts. The gene encoding human Hic-5 is located on chromosome 16, highlighting Hic-5′s significance in various cellular processes and potential implications in developmental biology and disease.
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Hic-5 Antibody (H-1) References:
- Hic-5 deficiency enhances mechanosensitive apoptosis and modulates vascular remodeling. | Kim-Kaneyama, JR., et al. 2011. J Mol Cell Cardiol. 50: 77-86. PMID: 20933520
- Hic-5 promotes invadopodia formation and invasion during TGF-β-induced epithelial-mesenchymal transition. | Pignatelli, J., et al. 2012. J Cell Biol. 197: 421-37. PMID: 22529104
- Role of Hic-5 in the formation of microvilli-like structures and the monocyte-endothelial interaction that accelerates atherosclerosis. | Arita-Okubo, S., et al. 2015. Cardiovasc Res. 105: 361-71. PMID: 25587044
- Hic-5 Mediates TGFβ-Induced Adhesion in Vascular Smooth Muscle Cells by a Nox4-Dependent Mechanism. | Fernandez, I., et al. 2015. Arterioscler Thromb Vasc Biol. 35: 1198-206. PMID: 25814672
- Hic-5 Regulates Actin Cytoskeletal Reorganization and Expression of Fibrogenic Markers and Myocilin in Trabecular Meshwork Cells. | Pattabiraman, PP. and Rao, PV. 2015. Invest Ophthalmol Vis Sci. 56: 5656-69. PMID: 26313302
- Hic-5 deficiency attenuates the activation of hepatic stellate cells and liver fibrosis through upregulation of Smad7 in mice. | Lei, XF., et al. 2016. J Hepatol. 64: 110-7. PMID: 26334580
- Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1. | Goreczny, GJ., et al. 2018. Oncogene. 37: 1699-1713. PMID: 29348458
- Hic-5 is required for activation of pancreatic stellate cells and development of pancreatic fibrosis in chronic pancreatitis. | Gao, L., et al. 2020. Sci Rep. 10: 19105. PMID: 33154390
- Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway. | Sha, L., et al. 2020. Aging (Albany NY). 12: 23598-23608. PMID: 33310972
- Hic-5 regulates extracellular matrix-associated gene expression and cytokine secretion in cancer associated fibroblasts. | Xu, W., et al. 2024. Exp Cell Res. 435: 113930. PMID: 38237846
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hic-5 Antibody (H-1) | sc-271447 | 200 µg/ml | $322.00 | |||
Hic-5 Antibody (H-1): m-IgG Fc BP-HRP Bundle | sc-540240 | 200 µg Ab; 10 µg BP | $361.00 | |||
Hic-5 Antibody (H-1): m-IgG1 BP-HRP Bundle | sc-541993 | 200 µg Ab; 20 µg BP | $361.00 | |||
Hic-5 (H-1) Neutralizing Peptide | sc-271447 P | 100 µg/0.5 ml | $69.00 | |||
Hic-5 Antibody (H-1) X | sc-271447 X | 200 µg/0.1 ml | $322.00 |