Hic-5 Antibody (C-6): sc-271353

Hic-5 Antibody (C-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Hic-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Hic-5 (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hic-5 protein, also known as transforming factor beta 1 induced transcript 1, plays a crucial role in cellular signaling and adhesion, as a member of the LIM family containing four LIM motifs and seven zinc finger domains. Localization to the nuclear matrix and focal adhesion complexes facilitates interactions with focal adhesions, which are essential for cell attachment and communication with the extracellular matrix. Hic-5′s ability to inhibit integrin-mediated cell spreading on fibronectin by competing with paxillin for focal adhesion kinase underscores Hic-5′s importance in regulating focal adhesion dynamics and downstream signaling pathways. Increased expression of Hic-5 can lead to cellular senescence in developing fibroblasts and block differentiation while inducing apoptosis in developing myoblasts, highlighting Hic-5′s dual role in both promoting and inhibiting cellular processes. Human Hic-5 gene location on chromosome 16 emphasizes Hic-5′s relevance in human biology and potential implications in various diseases.

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Hic-5 Antibody (C-6) References:

1. Lepidopteran DALP, and its mammalian ortholog HIC-5, function as negative regulators of muscle differentiation.  |  Hu, Y., et al. 1999. Proc Natl Acad Sci U S A. 96: 10218-23. PMID: 10468589
2. LIM domain protein Trip6 has a conserved nuclear export signal, nuclear targeting sequences, and multiple transactivation domains.  |  Wang, Y. and Gilmore, TD. 2001. Biochim Biophys Acta. 1538: 260-72. PMID: 11336797
3. Hic-5-reduced cell spreading on fibronectin: competitive effects between paxillin and Hic-5 through interaction with focal adhesion kinase.  |  Nishiya, N., et al. 2001. Mol Cell Biol. 21: 5332-45. PMID: 11463817
4. Hic-5 regulates epithelial to mesenchymal transition in ovarian cancer cells in a TGFβ1-independent manner.  |  Sheta, R., et al. 2017. Oncotarget. 8: 82506-82530. PMID: 29137281
5. Hic-5 deficiency attenuates hepatic ischemia reperfusion injury through TLR4/NF-κB signaling pathways.  |  Gao, L., et al. 2020. Life Sci. 249: 117517. PMID: 32147431
6. Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.  |  Wu, WS., et al. 2023. Am J Cancer Res. 13: 4903-4917. PMID: 37970347
7. Cloning from a mouse osteoblastic cell line of a set of transforming-growth-factor-beta 1-regulated genes, one of which seems to encode a follistatin-related polypeptide.  |  Shibanuma, M., et al. 1993. Eur J Biochem. 217: 13-9. PMID: 7901004
8. Characterization of the TGF beta 1-inducible hic-5 gene that encodes a putative novel zinc finger protein and its possible involvement in cellular senescence.  |  Shibanuma, M., et al. 1994. J Biol Chem. 269: 26767-74. PMID: 7929412
9. Induction of senescence-like phenotypes by forced expression of hic-5, which encodes a novel LIM motif protein, in immortalized human fibroblasts.  |  Shibanuma, M., et al. 1997. Mol Cell Biol. 17: 1224-35. PMID: 9032249
10. Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions.  |  Matsuya, M., et al. 1998. J Biol Chem. 273: 1003-14. PMID: 9422762
11. Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase.  |  Fujita, H., et al. 1998. J Biol Chem. 273: 26516-21. PMID: 9756887
12. Characterization of a focal adhesion protein, Hic-5, that shares extensive homology with paxillin.  |  Thomas, SM., et al. 1999. J Cell Sci. 112 (Pt 2): 181-90. PMID: 9858471

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hic-5 Antibody (C-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Hic-5 Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hic-5 Antibody (C-6) Specifications

Alternate Product Name: Hic-5

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 409-434

Epitope Target: Hic-5

Epitope Species: human

Product Reactivity/Detection: Hic-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Hic-5 Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent