Hic-5 Antibody (F-6): sc-137051
- Hic-5 Antibody (F-6) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 1-75 mapping at the N-terminus of Hic-5 of human origin
- recommended for detection of Hic-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- TransCruz reagent for ChIP application (sc-137051 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Hic-5 Antibody (F-6). This work is in progress.
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Hic-5 Antibody (F-6) is a mouse monoclonal IgG2b antibody that detects hydrogen-peroxide inducible clone 5 (Hic-5) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Hic-5 is a crucial member of the LIM family, characterized by four LIM motifs and seven zinc finger domains, which play a significant role in mediating protein-protein interactions within the cell. Hic-5 (F-6) antibody recognizes a protein primarily localized to the nuclear matrix and focal adhesion complexes, where Hic-5 is essential for regulating cellular adhesion and signaling pathways. Notably, Hic-5 inhibits integrin-mediated cell spreading on fibronectin by competing with paxillin for binding to focal adhesion kinase, thereby disrupting downstream signaling cascades vital for cell migration and proliferation. Hic-5 involvement extends to cellular senescence, particularly in developing fibroblasts, where increased expression can lead to growth arrest. During myogenesis, Hic-5 expression blocks differentiation and induces apoptosis in developing myoblasts, highlighting a regulatory role in muscle development. The gene encoding human Hic-5 is located on chromosome 16, underscoring evolutionary significance and potential implications in various biological processes.
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Hic-5 Antibody (F-6) References:
- Hydrogen peroxide-inducible clone 5 (Hic-5) as a potential therapeutic target for vascular and other disorders. | Kim-Kaneyama, JR., et al. 2012. J Atheroscler Thromb. 19: 601-7. PMID: 22472216
- Hic-5 is required for myofibroblast differentiation by regulating mechanically dependent MRTF-A nuclear accumulation. | Varney, SD., et al. 2016. J Cell Sci. 129: 774-87. PMID: 26759173
- Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase. | Dave, JM., et al. 2016. J Cell Sci. 129: 743-56. PMID: 26769900
- Hic-5 remodeling of the stromal matrix promotes breast tumor progression. | Goreczny, GJ., et al. 2017. Oncogene. 36: 2693-2703. PMID: 27893716
- Hic-5 regulates Src-induced invadopodia rosette formation and organization. | Gulvady, AC., et al. 2019. Mol Biol Cell. 30: 1298-1313. PMID: 30893012
- HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression. | Du, X., et al. 2019. Cell Death Dis. 10: 873. PMID: 31740661
- Hic-5 deficiency protects cerulein-induced chronic pancreatitis via down-regulation of the NF-κB (p65)/IL-6 signalling pathway. | Chen, H., et al. 2020. J Cell Mol Med. 24: 1488-1503. PMID: 31797546
- Suppressing of Src-Hic-5-JNK-AKT Signaling Reduced GAPDH Expression for Preventing the Progression of HuCCT1 Cholangiocarcinoma. | Wu, WS., et al. 2022. Pharmaceutics. 14: PMID: 36559193
- Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13. | Miyauchi, A., et al. 2023. Sci Rep. 13: 7446. PMID: 37156857
- Hic-5 antisense oligonucleotide inhibits advanced hepatic fibrosis and steatosis in vivo. | Noguchi, M., et al. 2024. JHEP Rep. 6: 101195. PMID: 39444410
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hic-5 Antibody (F-6) | sc-137051 | 200 µg/ml | $322.00 | |||
Hic-5 Antibody (F-6) X | sc-137051 X | 200 µg/0.1 ml | $322.00 |