HFE Antibody (C-12): sc-514405

HFE Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects HFE of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HFE (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HFE plays a crucial role in iron homeostasis by regulating iron absorption in the intestine, which is vital for preventing iron overload conditions such as hereditary hemochromatosis. This condition can lead to serious health issues, including liver cirrhosis, diabetes, skin hyperpigmentation, and heart failure. HFE is primarily localized in the crypt enterocytes of the duodenum, where HFE interacts with the transferrin receptor, forming a stable complex essential for iron transport. This intracellular localization allows HFE to effectively modulate iron uptake and release, thereby maintaining iron balance in the body. The gene encoding HFE is located on human chromosome 6p21.3, and proper HFE function is critical for preventing the development of preventable cancers associated with iron overload.

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HFE Antibody (C-12) References:

1. Role of HFE in iron metabolism, hereditary haemochromatosis, anaemia of chronic disease, and secondary iron overload.  |  Townsend, A. and Drakesmith, H. 2002. Lancet. 359: 786-90. PMID: 11888608
2. Human ferritin H and L sequences lie on ten different chromosomes.  |  McGill, JR., et al. 1987. Hum Genet. 76: 66-72. PMID: 3032771
3. Genes for the 'H' subunit of human ferritin are present on a number of human chromosomes.  |  Cragg, SJ., et al. 1985. Hum Genet. 71: 108-12. PMID: 3862645
4. Association of HFE protein with transferrin receptor in crypt enterocytes of human duodenum.  |  Waheed, A., et al. 1999. Proc Natl Acad Sci U S A. 96: 1579-84. PMID: 9990067

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HFE Antibody (C-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-HFE Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HFE Antibody (C-12) Specifications

Alternate Product Name: HFE

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 210-263

Epitope Target: HFE

Epitope Species: human

Product Reactivity/Detection: HFE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for HFE Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin