HEXIM2 Antibody (A-7): sc-390814

HEXIM2 Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects HEXIM2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). HEXIM2 (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HEXIM2 plays a crucial role in regulating transcription elongation by inhibiting the Positive Transcription Elongation Factor b (P-TEFb) complex, which is essential for proper expression of eukaryotic genes. P-TEFb, composed of catalytic subunit Cdk9 and regulatory subunits Cyclin T1 or T2, is vital for transitioning RNA polymerase II into the elongation phase of transcription. By binding to 7SK small nuclear ribonucleic acid particle, HEXIM2 helps maintain the balance of transcriptional activity, preventing excessive gene expression that could lead to cellular dysfunction. The interaction between HEXIM2 and HEXIM1, which also inhibits P-TEFb, highlights the importance of these proteins in gene regulation and their potential implications in various diseases, including cancer. HEXIM2 exhibits distinct expression patterns across different human tissues, suggesting involvement in tissue-specific regulatory mechanisms.

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HEXIM2 Antibody (A-7) References:

1. Compensatory contributions of HEXIM1 and HEXIM2 in maintaining the balance of active and inactive positive transcription elongation factor b complexes for control of transcription.  |  Yik, JH., et al. 2005. J Biol Chem. 280: 16368-76. PMID: 15713661
2. HEXIM2, a HEXIM1-related protein, regulates positive transcription elongation factor b through association with 7SK.  |  Byers, SA., et al. 2005. J Biol Chem. 280: 16360-7. PMID: 15713662
3. Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186.  |  Li, Q., et al. 2005. J Biol Chem. 280: 28819-26. PMID: 15965233
4. Inhibition of Tat activity by the HEXIM1 protein.  |  Fraldi, A., et al. 2005. Retrovirology. 2: 42. PMID: 15992410
5. Transcription-dependent association of multiple positive transcription elongation factor units to a HEXIM multimer.  |  Dulac, C., et al. 2005. J Biol Chem. 280: 30619-29. PMID: 15994294
6. siRNA depletion of 7SK snRNA induces apoptosis but does not affect expression of the HIV-1 LTR or P-TEFb-dependent cellular genes.  |  Haaland, RE., et al. 2005. J Cell Physiol. 205: 463-70. PMID: 16152622
7. The transcription-dependent dissociation of P-TEFb-HEXIM1-7SK RNA relies upon formation of hnRNP-7SK RNA complexes.  |  Barrandon, C., et al. 2007. Mol Cell Biol. 27: 6996-7006. PMID: 17709395
8. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated.  |  Krueger, BJ., et al. 2008. Nucleic Acids Res. 36: 2219-29. PMID: 18281698
9. Identification of HUGT1 as a potential BiP activator and a cellular target for improvement of recombinant protein production using a cDNA screening system.  |  Ku, SC., et al. 2009. Mol Cells. 27: 577-82. PMID: 19466607
10. 7SK snRNA: a noncoding RNA that plays a major role in regulating eukaryotic transcription.  |  Peterlin, BM., et al. 2012. Wiley Interdiscip Rev RNA. 3: 92-103. PMID: 21853533
11. [Comparison of analgesic effect between locally vinegar-processed preparation of fresh rhizoma Corydalis and traditionally vinegar-processed rhizoma Corydalis].  |  Liu, L., et al. 1990. Zhongguo Zhong Yao Za Zhi. 15: 666-7, 702. PMID: 2282156
12. Identification and validation of RNA-binding protein-related gene signature revealed potential associations with immunosuppression and drug sensitivity in glioma.  |  Chen, Z., et al. 2021. Cancer Med. 10: 7418-7439. PMID: 34482648

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HEXIM2 Antibody (A-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-HEXIM2 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HEXIM2 Antibody (A-7) Specifications

Alternate Product Name: HEXIM2

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-89

Epitope Target: HEXIM2

Epitope Species: human

Product Reactivity/Detection: HEXIM2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for HEXIM2 Antibody (A-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred HEXIM2 antibody is HEXIM2 Antibody (B-9): sc-398355.