HEXIM1 Antibody (G-10): sc-398479

HEXIM1 Antibody (G-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects HEXIM1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-HEXIM1 antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HEXIM1 plays a crucial role in regulating transcription by inhibiting the positive transcription elongation factor b (P-TEFb) complex, which is essential for the elongation phase of RNA polymerase II-mediated transcription. This inhibition occurs through HEXIM1′s association with the 7SK small nuclear ribonucleic acid particle, which is vital for maintaining proper gene expression levels. The P-TEFb complex, composed of the catalytic subunit Cdk9 and regulatory subunits Cyclin T1 or T2, is pivotal in controlling eukaryotic gene expression, and HEXIM1′s ability to modulate this complex underscores its importance in cellular processes. HEXIM1 and its homolog HEXIM2 can form stable homo- and hetero-oligomers, which may facilitate the assembly of the 7SK ribonucleoprotein complex, further emphasizing HEXIM1′s significance in transcriptional regulation. Despite their functional similarities, HEXIM1 and HEXIM2 exhibit distinct expression patterns across various cell lines and human tissues, highlighting the nuanced roles these proteins play in gene regulation.

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HEXIM1 Antibody (G-10) References:

1. Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters.  |  Kohoutek, J., et al. 2006. Proc Natl Acad Sci U S A. 103: 17349-54. PMID: 17088550
2. HMBA releases P-TEFb from HEXIM1 and 7SK snRNA via PI3K/Akt and activates HIV transcription.  |  Contreras, X., et al. 2007. PLoS Pathog. 3: 1459-69. PMID: 17937499
3. HEXIM1 controls satellite cell expansion after injury to regulate skeletal muscle regeneration.  |  Hong, P., et al. 2012. J Clin Invest. 122: 3873-87. PMID: 23023707
4. Cardiomyocyte-specific overexpression of HEXIM1 prevents right ventricular hypertrophy in hypoxia-induced pulmonary hypertension in mice.  |  Yoshikawa, N., et al. 2012. PLoS One. 7: e52522. PMID: 23300697
5. Phosphorylation of HEXIM1 at Tyr271 and Tyr274 Promotes Release of P-TEFb from the 7SK snRNP Complex and Enhances Proviral HIV Gene Expression.  |  Mbonye, UR., et al. 2015. Proteomics. 15: 2078-86. PMID: 25900325
6. HMBA is a putative HSP70 activator stimulating HEXIM1 expression that is down-regulated by estrogen.  |  Lama, R., et al. 2017. J Steroid Biochem Mol Biol. 168: 91-101. PMID: 28213333
7. HEXIM1 Diffusion in the Nucleus Is Regulated by Its Interactions with Both 7SK and P-TEFb.  |  Furlan, A., et al. 2019. Biophys J. 117: 1615-1625. PMID: 31590891
8. Inhibition of the histone demethylase, KDM5B, directly induces re-expression of tumor suppressor protein HEXIM1 in cancer cells.  |  Montano, MM., et al. 2019. Breast Cancer Res. 21: 138. PMID: 31805991
9. HEXIM1 controls P-TEFb processing and regulates drug sensitivity in triple-negative breast cancer.  |  Shao, H., et al. 2020. Mol Biol Cell. 31: 1867-1878. PMID: 32520633
10. Overexpression Bombyx mori HEXIM1 Facilitates Immune Escape of Bombyx mori Nucleopolyhedrovirus by Suppressing BmRelish-Driven Immune Responses.  |  Chen, G., et al. 2022. Viruses. 14: PMID: 36560640

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HEXIM1 Antibody (G-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HEXIM1 Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HEXIM1 Antibody (G-10) Specifications

Alternate Product Name: HEXIM1

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 207-230

Epitope Target: HEXIM1

Epitope Species: human

Product Reactivity/Detection: HEXIM1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HEXIM1 Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred HEXIM1 antibody is HEXIM1 Antibody (D-8): sc-390059.